Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi.
Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi.
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DOI:
10.1111/j.1365-2915.2012.01019.x
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发表时间:
2013-03
影响因子:
1.9
通讯作者:
Rafael MS
中科院分区:
文献类型:
--
作者:
Bridi LC;Sharakhova MV;Sharakhov IV;Cordeiro J;Azevedo Junior GM;Tadei WP;Rafael MS
Physical and genetic maps have been used for chromosomal localization of genes in vectors of infectious diseases. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to precisely map genes of interest. We report physical mapping of two actin genes on polytene chromosomes of the major malaria vector in Amazon Anopheles darlingi. The clones with the actin genes sequences were obtained from a cDNA library constructed from RNA isolated from adult females and males of An. darlingi. Each of the two clones was mapped to a unique site on the chromosomal arm 2L in subdivisions 21A (clone pl05-A04) and 23B (clone pl17-G06). The obtained results together with previous mapping data provide a suitable basis for comparative genomics and for establishing chromosomal homologies among major malaria vectors.
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DOI:
10.1073/pnas.1000251107
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影响因子:
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