Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi.

Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi.
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DOI:
10.1111/j.1365-2915.2012.01019.x
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发表时间:
2013-03
影响因子:
1.9
通讯作者:
Rafael MS
Rafael MS
中科院分区:
农林科学2区
文献类型:
--
作者:
Bridi LC;Sharakhova MV;Sharakhov IV;Cordeiro J;Azevedo Junior GM;Tadei WP;Rafael MS

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物理和遗传图谱已被用于传染病载体基因的染色体定位。疟疾蚊子中多染色体的可用性为精确定位感兴趣的基因提供了独特的机会。我们报告了两个肌动蛋白基因在亚马逊达林按蚊的主要疟疾媒介多染色体的物理定位。从成虫雌雄分离的RNA构建的cDNA文库中获得了含有肌动蛋白基因序列的克隆。darlingi。这两个克隆分别被定位到21A(克隆pl05-A04)和23B(克隆pl17-G06)染色体臂2L上的一个独特位点。获得的结果与先前的作图数据一起为比较基因组学和在主要疟疾媒介中建立染色体同源性提供了合适的基础。
Physical and genetic maps have been used for chromosomal localization of genes in vectors of infectious diseases. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to precisely map genes of interest. We report physical mapping of two actin genes on polytene chromosomes of the major malaria vector in Amazon Anopheles darlingi. The clones with the actin genes sequences were obtained from a cDNA library constructed from RNA isolated from adult females and males of An. darlingi. Each of the two clones was mapped to a unique site on the chromosomal arm 2L in subdivisions 21A (clone pl05-A04) and 23B (clone pl17-G06). The obtained results together with previous mapping data provide a suitable basis for comparative genomics and for establishing chromosomal homologies among major malaria vectors.
DOI: 10.1073/pnas.1000251107
发表时间: 2010-03-09
影响因子: 11.1
作者:
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