Expression of Cytokine mRNA in Bronchoalveolar Lavage Cells from Atopic Asthmatics before Late Antigen-Induced Reaction
Expression of Cytokine mRNA in Bronchoalveolar Lavage Cells from Atopic Asthmatics before Late Antigen-Induced Reaction
复制标题
晚期抗原诱导反应前特应性哮喘患者支气管肺泡灌洗细胞中细胞因子 mRNA 的表达
作者：
A. Miadonna;S. Gibelli;M. Lorini;A. Tedeschi;S. Oddera;G. Rossi;E. Crimi
Abstract. Recently, much attention has been given to the possible role of lymphocytes and their soluble products in causing and maintaining allergic inflammation. The aim of this study was to assess the production of mRNAs for interleukins (IL) in bronchoalveolar lavage (BAL) cells obtained from allergic asthmatics after challenge with the relevant allergen in the period between early and late reactions. We evaluated BAL fluid cells obtained from six asthmatic subjects and four nonatopic controls. Challenge was performed with the relevant allergen. BAL fluid cells were obtained by fiberoptic bronchoscopy and bronchoalveolar lavage. To detect mRNA encoding each cytokine in BAL cells we used a reverse transcriptase polymerase chain reaction method. We evaluated IL-1α, -2, -4, -5, -6, -13, and granulocyte-macrophage colony-stimulating factor (GM-CSF), and interferon-γ (IFN-γ). mRNAs for IL-1α, -2, -4, -5, and IFN-γ were detected in all of the atopic subjects; mRNAs for IL-6 and GM-CSF were found in five asthmatics; and mRNA for IL-13 was found in one patient only. In contrast, no mRNAs for IL-2, -4, -5, -6, -13, and GM-CSF were detected in the nonatopic healthy controls; mRNA for IL-1α was found in one out of four normal subjects; and mRNA for IFN-γ was evidenced in two of four subjects. The cellular environment in BAL fluids from allergic asthmatics before the clinical appearance of the late airway reaction shows an unrestricted expression of mRNA for cytokines. The local cytokine milieu could have an important role in the modulation of bronchial inflammation and in the appearance of allergic symptoms.
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DOI：
10.1164/ajrccm.149.2.8306027
发表时间：
1994-02-01
影响因子：
24.7
作者：
SIM, TC;GRANT, JA;ALAM, R
通讯作者：
ALAM, R
影响因子：
4.4
作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
通讯作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
DOI：
10.1164/ajrccm.153.4.8616572
发表时间：
1996-04-01
影响因子：
24.7
作者：
Alam, R;York, J;Ida, N
通讯作者：
Ida, N
过敏原诱导的鼻反应中趋化因子和其他细胞因子的分泌：局部类固醇治疗的抑制。
DOI：
10.1164/ajrccm.152.3.7545059
发表时间：
1995
期刊：
American journal of respiratory and critical care medicine.
影响因子：
--
作者：
Sim,TC;Reece,LM;Hilsmeier,KA;Grant,JA;Alam,R
通讯作者：
Alam,R
抗原激发后数小时从鼻子获得的含组胺细胞具有嗜碱性粒细胞的功能和表型特征。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Iliopoulos,O;Baroody,FM;Naclerio,RM;Bochner,BS;Kagey-Sobotka,A;Lichtenstein,LM
通讯作者：
Lichtenstein,LM