Cytokinin receptor CRE1 is required for the defense response of Nicotiana tabacum to Chilli veinal mottle virus
Cytokinin receptor CRE1 is required for the defense response of Nicotiana tabacum to Chilli veinal mottle virus
复制标题
烟草对辣椒静脉斑驳病毒的防御反应需要细胞分裂素受体 CRE1
DOI：
10.1007/s10725-020-00579-3
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发表时间：
2020-01
影响因子：
4.2
通讯作者：
Xi Dehui
中科院分区：
文献类型：
--
作者：
Zou Wenshan;Chen Lijuan;Zou Jialing;Han Hongyan;Fei Chunyan;Lin Honghui;Xi Dehui
细胞分裂素作为一种重要的植物激素，赋予植物对几种真菌和细菌病原体的抗性。然而，细胞分裂素在烟草病毒抗性中的发现鲜有报道。在本研究中，我们利用重组pRNAi-LIC-CRE1，通过农杆菌介导转化获得了烟草细胞分裂素受体1 （CRE1）缺陷突变体。结果表明，CRE1基因缺失增加了烟草对辣椒斑驳病毒（ChiVMV）感染的易感性。与野生型（WT）相比，CRE1缺陷突变株表现出更明显的chivmv诱导症状和更高的病毒外壳蛋白基因转录水平。接种病毒后，CRE1缺陷植株抗氧化代谢发生改变。病毒对CRE1缺陷突变体植株的光系统损害也比WT植株严重。此外，在CRE1缺失的植物中，受ChiVMV感染诱导的几种防御相关基因的表达受到抑制。综上所述，这些结果表明，CRE1是烟草对hiv感染的防御反应所必需的。
As an important phytohormone, cytokinins confer resistance of plants to several fungal and bacterial pathogens. However, the discovery of cytokinins in the viral resistance of Nicotiana tabacum is seldom reported. In the current study, we obtained tobacco CRE1 (cytokinin receptor 1) deficient mutants by Agrobacterium-mediated transformation utilizing the recombinant pRNAi-LIC-CRE1. Our conclusions indicated that CRE1 deficiency increased the susceptibility of N. tabacum to Chilli veinal mottle virus (ChiVMV) infection. The CRE1 deficient mutant plants displayed both more evident ChiVMV-induced symptom and higher transcription level of viral coat protein gene compared to the wild type (WT) plants. The antioxidant metabolism was altered in CRE1 deficient plants under virus inoculation. Photosystem damages caused by virus were also more severe in CRE1 deficient mutant plants compared to that in the WT plants. Additionally, expressions of several defense-related genes induced by ChiVMV infection were repressed in CRE1 deficient plants. Taken all together, these results suggested that CRE1 is required for N. tabacum defense responses to ChiVMV infection.
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