Physiological and Transcriptional Responses of Industrial Rapeseed (Brassica napus) Seedlings to Drought and Salinity Stress
Physiological and Transcriptional Responses of Industrial Rapeseed (Brassica napus) Seedlings to Drought and Salinity Stress
复制标题
工业油菜（甘蓝型油菜）幼苗对干旱和盐胁迫的生理和转录反应
DOI：
10.3390/ijms20225604
复制
发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
梁明祥
中科院分区：
文献类型：
--
作者：
王吉;焦娇;周梦佳;金泽阳;于永健;梁明祥
非生物胁迫极大地抑制作物生长并降低产量。然而，很少有人知道的转录组的变化，发生在工业油料作物，油菜（甘蓝型油菜），在响应非生物胁迫。在这项研究中，我们研究了油菜对干旱（模拟处理与15%（w/v）聚乙二醇（PEG）6000）和盐（150 mM NaCl）胁迫的生理和转录反应。处理3 h后，幼苗中脯氨酸含量在两种条件下都大大增加，而抗氧化酶的水平保持不变。我们组装了来自油菜叶片和根的转录本，并首次对油菜基因组数据库进行了BLACKS搜索。基因本体分析表明，参与催化活性、代谢过程和刺激响应的DEG高度富集。京都基因和基因组百科全书（KEGG）途径分析显示，来自代谢途径和次级代谢物生物合成类别的差异表达基因（DEG）高度富集。我们确定成髓细胞瘤（MYB），NAM/ATAF 1 -2/CUC 2（NAC），和APETALA 2/乙烯反应元件结合蛋白（AP 2-EREBP）转录因子作为控制下游基因表达的主要开关发挥作用，脯氨酸在短期非生物胁迫处理下由于合成表达增加和降解表达减少而发挥作用。此外，许多共同的基因功能在这两种类型的压力在这油菜。
Abiotic stress greatly inhibits crop growth and reduces yields. However, little is known about the transcriptomic changes that occur in the industrial oilseed crop, rapeseed (Brassica napus), in response to abiotic stress. In this study, we examined the physiological and transcriptional responses of rapeseed to drought (simulated by treatment with 15% (w/v) polyethylene glycol (PEG) 6000) and salinity (150 mM NaCl) stress. Proline contents in young seedlings greatly increased under both conditions after 3 h of treatment, whereas the levels of antioxidant enzymes remained unchanged. We assembled transcripts from the leaves and roots of rapeseed and performed BLASTN searches against the rapeseed genome database for the first time. Gene ontology analysis indicated that DEGs involved in catalytic activity, metabolic process, and response to stimulus were highly enriched. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that differentially expressed genes (DEGs) from the categories metabolic pathways and biosynthesis of secondary metabolites were highly enriched. We determined that myeloblastosis (MYB), NAM/ATAF1-2/CUC2 (NAC), and APETALA2/ethylene-responsive element binding proteins (AP2-EREBP) transcription factors function as major switches that control downstream gene expression and that proline plays a role under short-term abiotic stress treatment due to increased expression of synthesis and decreased expression of degradation. Furthermore, many common genes function in the response to both types of stress in this rapeseed.
登录
查看更多内容
影响因子：
7.4
作者：
Aurine Verkest;M. Byzova;Cindy Martens;P. Willems;T. Verwulgen;Bram Slabbinck;D. Rombaut;Jan Van de Velde;K. Vandepoele;E. Standaert;Marrit Peeters;M. Van Lijsebettens;F. Van Breusegem;M. De Block
通讯作者：
Aurine Verkest;M. Byzova;Cindy Martens;P. Willems;T. Verwulgen;Bram Slabbinck;D. Rombaut;Jan Van de Velde;K. Vandepoele;E. Standaert;Marrit Peeters;M. Van Lijsebettens;F. Van Breusegem;M. De Block
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
5.6
作者：
Zhao G;Zhao Y;Yu X;Kiprotich F;Han H;Guan R;Wang R;Shen W
通讯作者：
Shen W
影响因子：
3.7
作者：
Long W;Zou X;Zhang X
通讯作者：
Zhang X
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y