Ultrasensitive detection of 3D cerebral microvascular network dynamics in vivo.
Ultrasensitive detection of 3D cerebral microvascular network dynamics in vivo.
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DOI：
10.1016/j.neuroimage.2014.08.051
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发表时间：
2014-12
期刊：
影响因子：
5.7
通讯作者：
Du C
中科院分区：
文献类型：
--
作者：
Pan Y;You J;Volkow ND;Park K;Du C
Despite widespread applications of multiphoton microscopy in microcirculation, its small field of view and inability to instantaneously quantify cerebral blood flow velocity (CBFv) in vascular networks limit its utility in investigating the heterogeneous responses to brain stimulations. Optical Doppler tomography (ODT) provides 3D images of CBFv networks, but it suffers poor sensitivity for measuring capillary flows. Here we report a new method, contrast-enhanced ODT with intralipid that significantly improves quantitative CBFv imaging of capillary networks by obviating the errors from long latency between flowing red blood cells (low hematocrit ~20% in capillaries). This enhanced sensitivity allowed us to measure the ultraslow microcirculation surrounding a brain tumor and the abnormal ingrowth of capillary flows in the tumor as well as in ischemia triggered by chronic cocaine in the mouse brain that could not be detected by regular ODT. It also enabled significantly enhanced sensitivity for quantifying the heterogeneous CBFv responses of vascular networks to acute cocaine. Inasmuch as intralipids are widely used for parenteral nutrition the intralipid contrast method has translational potential for clinical applications.
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