3D-printed assistive pipetting system for gel electrophoresis for technicians with low acuity vision.
3D-printed assistive pipetting system for gel electrophoresis for technicians with low acuity vision.
复制标题
用于凝胶电泳的3d打印辅助移液系统，适用于视力较差的技术人员。
DOI：
10.2144/btn-2020-0139
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发表时间：
2021-01
期刊：
影响因子：
2.7
通讯作者：
Buhr ED
中科院分区：
文献类型：
--
作者：
Huynh AV;Stein P;Buhr ED
在分子生物学实验室中，许多任务需要精细的运动控制和高敏锐度的视觉。例如，有视力障碍的实验室技术人员在将样品加载到水平琼脂糖凝胶的小威尔斯孔中时遇到困难。我们开发了一种3D打印的凝胶加载系统，允许低对比度视觉的技术人员正确加载凝胶。它包括铸造托盘、桥和改进的梳子。该系统提供高对比度视野以提高可见性，并且桥允许移液器吸头插入正确的位置且仅插入正确的深度。打印该设备所需的计算机文件免费提供，以增加分子生物学实验室对视力障碍者的可及性。我们已经为视力障碍的人生产了一个系统，可以成功地加载琼脂糖凝胶进行凝胶电泳。该系统可以使用3D打印机生产。有三个组成部分：铸造托盘、桥和改进的梳。桥装配在铸造托盘的侧凹口中并悬挂在托盘上方。梳子插入到桥，而熔融凝胶倾倒。当凝胶冷却时，移除梳子，将桥留在原位。然后，移液器只能插入正确的位置和正确的深度，以加载威尔斯孔。重要的是，桥和托盘是对比色，以帮助视觉识别。
In molecular biology laboratories, many tasks require fine motor control and high acuity vision. For example, lab technicians with visual impairment experience difficulty loading samples into the small wells of a horizontal agarose gel. We have developed a 3D-printable gel loading system which allows technicians with low-contrast vision to load gels correctly. It includes a casting tray, a bridge, and a modified comb. The system provides a high-contrast visual field to improve visibility, and the bridge allows pipette tips to be inserted at the correct location and only to the correct depth. The necessary computer files for printing this device are freely available to increase the accessibility of molecular biology laboratories to people with visual impairment. We have produced a system for people with visual impairment to successfully load an agarose gel for gel electrophoresis. This system can be produced using a 3D printer. There are three components: a casting tray, a bridge, and a modified comb. The bridge fits into side notches in the casting tray and is suspended above the tray. The comb is inserted into the bridge while the molten gel is poured. When the gel cools, the comb is removed leaving the bridge in place. Pipettes can then only be inserted in the correct location and to the correct depth to load the wells. Importantly, the bridge and tray are contrasting colors to aid in visual discernment.
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影响因子：
11
作者：
Cheng, Liang;Wang, Yi;Cui, Wenguo
通讯作者：
Cui, Wenguo
影响因子：
4.1
作者：
Tiselius, A
通讯作者：
Tiselius, A
DOI：
10.1016/s0021-9673(01)84276-x
发表时间：
1989-10-20
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
VESTERBERG, O
通讯作者：
VESTERBERG, O
影响因子：
4.1
作者：
SMITHIES, O
通讯作者：
SMITHIES, O