Daughter centrioles assemble preferentially towards the nuclear envelope in Drosophila syncytial embryos.
Daughter centrioles assemble preferentially towards the nuclear envelope in Drosophila syncytial embryos.
复制标题
DOI：
10.1098/rsob.210343
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发表时间：
2022-01
期刊：
影响因子：
5.8
通讯作者：
Conduit PT
中科院分区：
文献类型：
--
作者：
Cunningham NHJ;Bouhlel IB;Conduit PT
中心体是动物细胞中重要的微管组织体。它们由一对中心粒组成，中心粒被中心粒周围物质包围，中心粒形成微管并组织微管。为了维持中心体数量，中心粒必须在每个细胞周期内复制一次且仅复制一次。在s期，一个新的“子”中心粒在每个径向对称的“母”中心粒的一侧正交地形成。复制的错误调控可能导致在单个母中心粒周围同时形成多个子中心粒，导致中心体扩增，这是癌症的一个标志。目前尚不清楚单个复制位点是如何建立的。目前还不清楚这个位点是预先定义的还是随机定位在母中心粒周围。在这里，我们发现在果蝇合胞胚胎中，子中心粒优先聚集在面对核膜的母体一侧，中心体紧密附着在核膜上。这种位置偏好在复制早期建立，并在子中心粒组装过程中保持稳定，但在中心体被迫失去与核膜的连接时丢失。这表明非中心体线索影响中心粒复制，并提出了这些外部线索可能有助于建立单个复制位点的可能性。
Centrosomes are important organizers of microtubules within animal cells. They comprise a pair of centrioles surrounded by the pericentriolar material, which nucleates and organizes the microtubules. To maintain centrosome numbers, centrioles must duplicate once and only once per cell cycle. During S-phase, a single new ‘daughter’ centriole is built orthogonally on one side of each radially symmetric ‘mother’ centriole. Mis-regulation of duplication can result in the simultaneous formation of multiple daughter centrioles around a single mother centriole, leading to centrosome amplification, a hallmark of cancer. It remains unclear how a single duplication site is established. It also remains unknown whether this site is pre-defined or randomly positioned around the mother centriole. Here, we show that within Drosophila syncytial embryos daughter centrioles preferentially assemble on the side of the mother facing the nuclear envelope, to which the centrosomes are closely attached. This positional preference is established early during duplication and remains stable throughout daughter centriole assembly, but is lost in centrosomes forced to lose their connection to the nuclear envelope. This shows that non-centrosomal cues influence centriole duplication and raises the possibility that these external cues could help establish a single duplication site.
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影响因子：
9.2
作者：
Conduit, Paul T.;Brunk, Kathrin;Raff, Jordan W.
通讯作者：
Raff, Jordan W.
影响因子：
64.8
作者：
Dzhindzhev, Nikola S.;Yu, Quan D.;Glover, David M.
通讯作者：
Glover, David M.
影响因子：
7.7
作者：
Conduit, Paul T.;Wainman, Alan;Raff, Jordan W.
通讯作者：
Raff, Jordan W.
DOI：
10.1083/jcb.201107086
发表时间：
2011-10-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brownlee CW;Klebba JE;Buster DW;Rogers GC
通讯作者：
Rogers GC
影响因子：
3.8
作者：
Denu RA;Zasadil LM;Kanugh C;Laffin J;Weaver BA;Burkard ME
通讯作者：
Burkard ME