Fusion-pore expansion during syncytium formation is restricted by an actin network.
Fusion-pore expansion during syncytium formation is restricted by an actin network.
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DOI：
10.1242/jcs.032169
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发表时间：
2008-11-01
影响因子：
4
通讯作者：
Chernomordik LV
中科院分区：
文献类型：
--
作者：
Chen A;Leikina E;Melikov K;Podbilewicz B;Kozlov MM;Chernomordik LV
在动物发育和病理生理学中的细胞-细胞融合涉及由蛋白质融合剂形成的新生融合孔的扩张，以产生细胞大小直径的开放腔。在这里，我们探讨了在合胞体的形成，这是由一个良好的特征融合杆状病毒gp 64启动的微米级孔的扩大。单个或更常见的多个熔合孔的径向膨胀在紧密接触区中进行而不损失膜材料。孔的生长需要细胞代谢，并伴随着孔下肌动蛋白皮质的局部分解。肌动蛋白修饰剂的作用表明，肌动蛋白皮质减缓了孔扩张。我们提出，强烈弯曲的融合孔边缘的生长受到肌动蛋白网络的动态阻力的限制，并由参与产生高度弯曲的细胞内膜隔室的膜弯曲蛋白驱动。
Cell-cell fusion in animal development and in pathophysiology involves expansion of nascent fusion pores formed by protein fusogens to yield an open lumen of cell-size diameter. Here we explored the enlargement of micron-scale pores in syncytium formation, which was initiated by a well-characterized fusogen baculovirus gp64. Radial expansion of a single or, more often, of multiple fusion pores proceeds without loss of membrane material in the tight contact zone. Pore growth requires cell metabolism and is accompanied by a local disassembly of the actin cortex under the pores. Effects of actin-modifying agents indicate that the actin cortex slows down pore expansion. We propose that the growth of the strongly bent fusion-pore rim is restricted by a dynamic resistance of the actin network and driven by membrane-bending proteins that are involved in the generation of highly curved intracellular membrane compartments.
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DOI：
10.1083/jcb.109.6.2703
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Griffiths G;Back R;Marsh M
通讯作者：
Marsh M
影响因子：
2.7
作者：
KEMPF, C;KOHLER, U;KOBLET, H
通讯作者：
KOBLET, H
影响因子：
7.5
作者：
Antonny, Bruno
通讯作者：
Antonny, Bruno
影响因子：
3.4
作者：
CHERNOMORDIK, LV;SOWERS, AE
通讯作者：
SOWERS, AE
影响因子：
3.3
作者：
Gattegno, Tamar;Mittal, Aditya;Podbilewicz, Benjamin
通讯作者：
Podbilewicz, Benjamin