A Sec-Dependent Secretory Protein of the Huanglongbing-Associated Pathogen Suppresses Hypersensitive Cell Death in Nicotiana benthamiana.
A Sec-Dependent Secretory Protein of the Huanglongbing-Associated Pathogen Suppresses Hypersensitive Cell Death in Nicotiana benthamiana.
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DOI：
10.3389/fmicb.2020.594669
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发表时间：
2020
影响因子：
5.2
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Zhang C;Du P;Yan H;Zhu Z;Wang X;Li W
柑橘黄龙病病原菌是一种革兰氏阴性细菌，主要分布于韧皮部，是柑橘黄龙病的病原菌。在这项研究中，我们确定了CLas编码的Sec依赖性分泌蛋白CLIBASIA_04405，可能有助于该细菌的致病性。CLIBASIA_04405基因在柑橘中的表达水平显著高于木虱。本氏烟草叶片中的成熟CLIBASIA_04405蛋白（m4405）的瞬时过表达可以抑制由小鼠BAX和致病疫霉INF 1触发的基于过敏反应（HR）的细胞死亡和H2 O2积累。丙氨酸取代诱变试验揭示了氨基酸簇EKR 45 -47和DE 64 -65在细胞死亡抑制中的重要性。对转基因烟草进行挑战接种。本氏菌表达的m4405与假单胞菌DC 3000 Δ hopQ 1 -1的共培养证明细菌增殖大大降低。转录组分析和RT-qPCR分析表明，转基因m4405中6种小分子热激蛋白（small heat shock proteins，sHSPs）的基因表达量显著高于野生型N.本萨米亚纳此外，转基因m4405株系表现出矮化和叶片变形的表型。这些数据表明，m4405是CLas的毒力因子。
“Candidatus Liberibacter asiaticus” (CLas) is a phloem-restricted Gram-negative bacterium that is the causal agent of citrus huanglongbing (HLB). In this study, we identified a CLas-encoded Sec-dependent secretory protein CLIBASIA_04405 that could contribute to the pathogenicity of this bacterium. The gene expression level of CLIBASIA_04405 was significantly higher in citrus than in psyllids. Transient overexpression of the mature CLIBASIA_04405 protein (m4405) in Nicotiana benthamiana leaves could suppress hypersensitive response (HR)-based cell death and H2O2 accumulation triggered by the mouse BAX and the Phytophthora infestans INF1. An alanine-substitution mutagenesis assay revealed the essential of amino acid clusters EKR45–47 and DE64–65 in cell death suppression. Challenge inoculation of the transgenic N. benthamiana-expressing m4405 with Pseudomonas syringae DC3000ΔhopQ1-1 demonstrated the greatly reduced bacterial proliferation. Remarkably, transcriptome profiling and RT-qPCR analysis disclosed that the gene expression of six small heat shock proteins (sHSPs), a set of plant defense regulators, were significantly elevated in the transgenic m4405 lines compared with those in wild-type N. benthamiana. In addition, the transgenic m4405 lines displayed phenotypes of dwarfism and leaf deformation. Altogether, these data indicated that m4405 was a virulence factor of CLas.
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影响因子：
16.6
作者：
Clark K;Franco JY;Schwizer S;Pang Z;Hawara E;Liebrand TWH;Pagliaccia D;Zeng L;Gurung FB;Wang P;Shi J;Wang Y;Ancona V;van der Hoorn RAL;Wang N;Coaker G;Ma W
通讯作者：
Ma W
影响因子：
3.5
作者：
Jain, Mukesh;Fleites, Laura A.;Gabriel, Dean W.
通讯作者：
Gabriel, Dean W.
影响因子：
3.7
作者：
Bricchi I;Bertea CM;Occhipinti A;Paponov IA;Maffei ME
通讯作者：
Maffei ME
影响因子：
7.2
作者：
Bos, Jorunn I. B.;Kanneganti, Thirumala-Devi;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
5.6
作者：
Haslbeck, Martin;Vierling, Elizabeth
通讯作者：
Vierling, Elizabeth