Near-infrared laser delivery of nanoparticles to developing embryos: a study of efficacy and viability.
Near-infrared laser delivery of nanoparticles to developing embryos: a study of efficacy and viability.
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DOI：
10.1002/biot.201000205
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发表时间：
2011-05
影响因子：
4.7
通讯作者：
Khodaparast, Giti A.
中科院分区：
文献类型：
--
作者：
Umanzor-Alvarez, Jose;Wade, Emily C.;Gifford, Aliya;Nontapot, Kankowan;Cruz-Reese, Ariana;Gotoh, Tetsuya;Sible, Jill C.;Khodaparast, Giti A.
靶向递送材料到单个细胞仍然是纳米科学和纳米医学的一个挑战。近红外（NIR）激光注射可能是一种有前途的替代人工注射（微吸管直径限制瞄准小细胞）或其他激光技术（如皮秒绿色和紫外线激光，这可能会损害细胞）。然而，近红外脉冲传递纳米粒子的效率和对活细胞的任何不利影响需要彻底的测试。为此，我们研究了近红外激光注射将量子点（QDs）注入非洲爪蟾胚胎细胞的效果和毒性。由于该模型系统不仅提供活细胞，而且还提供发育中的生物体，因此我们能够评估近红外脉冲对胚胎发育（通过蝌蚪阶段）的相对长期影响。我们开发了不影响胚胎活力或形态的近红外脉冲参数，并像手动注射一样有效地递送量子点。高强度的近红外脉冲对目标细胞造成永久性损伤，因此近红外脉冲也可能被证明对组织内特定细胞的消融有用。
Targeted delivery of materials to individual cells remains a challenge in nanoscience and nanomedicine. Near infrared (NIR) laser injection may be a promising alternative to manual injection (where the micropipet diameter limits targeting to small cells) or other laser techniques (such as picosecond green and UV lasers, which can be damaging to cells). However, the efficiency with which NIR pulses can deliver nanoparticles and any adverse effects on living cells needs thorough testing. Toward this end, we have determined the efficacy and toxicity of delivering quantum dots (QDs) into cells of Xenopus laevis embryos by NIR laser injection. Because this model system provides not only living cells but also a developing organism, we were able to assess relatively long-term effects of NIR pulses on embryonic development (through the tadpole stage). We developed parameters for NIR pulses that did not affect embryonic viability or morphology and delivered QDs as effectively as manual injection. Higher intensities of NIR pulses caused permanent damage to the targeted cells, and thus NIR pulses may also prove useful for ablation of specific cells within tissues.
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