Dynamic glucose enhanced MRI of the placenta in a mouse model of intrauterine inflammation.
Dynamic glucose enhanced MRI of the placenta in a mouse model of intrauterine inflammation.
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DOI：
10.1016/j.placenta.2018.07.012
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发表时间：
2018-09
期刊：
影响因子：
3.8
通讯作者：
Burd I
中科院分区：
文献类型：
--
作者：
Wu D;Xu J;Lei J;Mclane M;van Zijl PC;Burd I
我们研究了动态葡萄糖增强（DGE）MRI评估宫内炎性损伤（IUI）小鼠模型胎盘功能的可行性。DGE使用葡萄糖化学交换饱和转移（glucoCEST）效应来反映输注的D-葡萄糖。在妊娠CD 1小鼠中通过在胚胎第17天宫内注射脂多糖（LPS）诱导IUI。在损伤后6小时在11.7T扫描仪上进行体内MRI，并使用共振可变延迟多脉冲（onVELT）技术测量glucoCEST效应。在25分钟的时间内重复onVibration采集，并在开始后5分钟输注D-葡萄糖。使用onVDP标记和未标记图像之间的归一化信号差（ΔSN）表征时间分辨glucoCEST信号。暴露于PBS的胎盘（n=6）中的ΔSN在输注后1-3分钟之间显示出初始下降，随后在5-20分钟之间出现正峰值，预计该时间段与葡萄糖摄取和转运过程相关。暴露于LPS的胎盘（n=10）中，阳性峰减少或甚至消失，相应的曲线下面积（AUC）显著低于对照组。特别是，AUC图表明胎盘胎儿侧存在显著的组间差异。我们还发现，葡萄糖转运蛋白1在脂多糖暴露的胎盘没有回应母体葡萄糖的挑战。DGE-MRI可用于评估与葡萄糖利用相关的胎盘功能。该技术使用无毒的可生物降解的试剂（D-葡萄糖），因此具有快速转化为人类胎盘疾病研究的潜力。
We investigated the feasibility of dynamic glucose enhanced (DGE) MRI in accessing placental function in a mouse model of intrauterine inflammatory injury (IUI). DGE uses the glucose chemical exchange saturation transfer (glucoCEST) effect to reflect infused D-glucose. IUI was induced in pregnant CD1 mice by intrauterine injection of lipopolysaccharide (LPS) on embryonic day 17. In vivo MRI was performed on an 11.7T scanner at 6hrs after injury, and glucoCEST effect was measured using an on- resonance variable delay multi-pulse (onVDMP) technique. onVDMP acquisition was repeated over a period of 25 min, and D-glucose was infused 5 minutes after the start. The time-resolved glucoCEST signals were characterized using the normalized signal difference (ΔSN ) between onVDMP-labeled and nonlabeled images. ΔSN in the PBS-exposed placentae (n=6) showed an initial drop between 1–3 minutes after infusion, followed by a positive peak between 5–20 minutes, the time period expected to be associated with the process of glucose uptake and transport. In the LPS-exposed placentae (n=10), the positive peak was reduced or even absent, and the corresponding area-under-the-curve (AUC) was significantly lower than that in the controls. Particularly, the AUC maps suggested prominent group differences in the fetal side of the placenta. We also found that glucose transporter 1 in the LPS-exposed placentae did not respond to maternal glucose challenge. DGE-MRI is useful for evaluating placental functions related to glucose utilization. The technique uses a non-toxic biodegradable agent (D-glucose) and thus has a potential for rapid translation to human studies of placental disorders.
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影响因子：
3.3
作者：
Huen, Isaac;Morris, David M.;Naish, Josephine H.
通讯作者：
Naish, Josephine H.
DOI：
10.1097/00004647-199605000-00009
发表时间：
1996-05-01
影响因子：
6.3
作者：
Gruetter, R;Novotny, EJ;Shulman, RG
通讯作者：
Shulman, RG
DOI：
10.1111/1471-0528.12804
发表时间：
2014-12-01
影响因子：
5.8
作者：
Huen, I.;Morris, D. M.;Johnstone, E. D.
通讯作者：
Johnstone, E. D.
影响因子：
3.6
作者：
HAUGUEL, S;DESMAIZIERES, V;CHALLIER, JC
通讯作者：
CHALLIER, JC
影响因子：
3.3
作者：
Chan, Kannie W. Y.;McMahon, Michael T.;Kato, Yoshinori;Liu, Guanshu;Bulte, Jeff W. M.;Bhujwalla, Zaver M.;Artemov, Dmitri;van Zijl, Peter C. M.
通讯作者：
van Zijl, Peter C. M.