Coextruded, aligned, and gradient-modified poly(ε-caprolactone) fibers as platforms for neural growth.
Coextruded, aligned, and gradient-modified poly(ε-caprolactone) fibers as platforms for neural growth.
复制标题
共同进行，排列和梯度修饰的聚（ε-二甲酰胺）纤维作为神经生长的平台。
DOI：
10.1021/bm501767x
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发表时间：
2015-03-09
影响因子：
6.2
通讯作者：
Pokorski JK
中科院分区：
文献类型：
--
作者：
Kim SE;Harker EC;De Leon AC;Advincula RC;Pokorski JK
聚合物纤维对再生医学越来越感兴趣，因为由这些纤维制成的材料是多孔的，允许细胞渗透，营养物质流入和废物流出。近年来，多层共挤出技术已成为一种可扩展的快速制造方法，可生产微米级至亚微米级的纤维。在这份报告中，我们描述了多层共挤出的排列聚（ε-己内酯）（PCL）纤维，然后通过一个简单的光化学图案化，以创建表面固定的梯度到聚合物纤维。使用梯度光掩模以控制光源强度，用炔丙基二苯甲酮的线性梯度光化学修饰PCL纤维。然后，侧链炔能够与叠氮化物修饰的IKVAV肽进行铜催化的叠氮化物-炔环加成反应，以进一步官能化表面。使用在纤维上培养的PC-12细胞，评价经修饰的IKVAV纤维的神经细胞粘附和神经分化。通过细胞伸长、神经突分化和定向观察到，排列的梯度纤维为神经突生长和神经细胞排列提供了方向线索。本文提出的工作描述了一种可扩展的纤维系统，其与简单的化学图案化相结合，以产生具有受控表面梯度的对齐纤维作为细胞接种支架。
Polymeric fibers are of increasing interest to regenerative medicine, as materials made from these fibers are porous, allowing for cell infiltration, influx of nutrients, and efflux of waste products. Recently, multilayered coextrusion has emerged as a scalable and rapid fabrication method to yield microscale to submicron fibers. In this report, we describe the multilayered coextrusion of aligned poly(ε-caprolactone) (PCL) fibers, followed by a simple photochemical patterning to create surface-immobilized gradients onto the polymer fibers. PCL fibers were photochemically decorated with a linear gradient of propargyl benzophenone using a gradient photomask to control light source intensity. The pendant alkynes were then able to undergo the copper-catalyzed azide–alkyne cycloaddition reaction with an azide-modified IKVAV peptide to further functionalize the surface. Gradient-modified IKVAV fibers were evaluated for neural cell adhesion and neural differentiation, using PC-12 cells cultured onto the fibers. The aligned gradient fibers provided directional cues for neurite outgrowth and alignment of neural cells, as observed by cellular elongation, neurite differentiation, and orientation. The work presented herein describes a scalable fiber system combined with simple chemical patterning to generate aligned fibers with controlled surface gradients as cell-seeding scaffolds.
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影响因子：
14
作者：
Callahan, Laura A. Smith;Xie, Sibai;Becker, Matthew L.
通讯作者：
Becker, Matthew L.
影响因子：
5.3
作者：
Mai, Junyu;Fok, Lee;Poo, Mu-ming
通讯作者：
Poo, Mu-ming
影响因子：
9.5
作者：
Mattanavee, Waradda;Suwantong, Orawan;Supaphol, Pitt
通讯作者：
Supaphol, Pitt
影响因子：
5.6
作者：
Sivolella S;Brunello G;Ferrarese N;Della Puppa A;D'Avella D;Bressan E;Zavan B
通讯作者：
Zavan B
DOI：
10.1002/neu.20075
发表时间：
2005-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Adams, DN;Kao, EYC;Letourneau, PC
通讯作者：
Letourneau, PC