Gold Nanotapes and Nanopinecones in a Quantitative Lateral Flow Assay for the Cancer Biomarker Carcinoembryonic Antigen.
Gold Nanotapes and Nanopinecones in a Quantitative Lateral Flow Assay for the Cancer Biomarker Carcinoembryonic Antigen.
复制标题
DOI：
10.1021/acsanm.3c03053
复制
发表时间：
2023-10-13
影响因子：
5.9
通讯作者：
Evans, Stephen D.
中科院分区：
文献类型：
--
作者：
Fox, Joseph;Batchelor, Damien V. B.;Roberts, Holly;Moorcroft, Samuel C. T.;Valleley, Elizabeth M. A.;Coletta, Patricia Louise;Evans, Stephen D.
关键词：
结直肠癌是全球第三大常见恶性肿瘤，也是癌症死亡的第二大原因。多项研究表明，患者血清中癌胚抗原水平与疾病预后不良有关。因此，检测低水平癌胚抗原的能力可应用于早期疾病诊断、评估和复发监测。现有的癌胚抗原检测方法通常需要多种试剂、训练有素的操作人员或复杂的程序。缓解这些问题的一种方法是侧流分析，这是一种基于纸的平台，可以检测和定量复杂混合物中的目标分析物。该测试是快速的，是即时的，具有较长的保质期，并可以在环境条件下储存，使它们成为在一系列环境中使用的理想选择。虽然侧流检测通常使用球形金纳米颗粒来产生经典的红色信号，但最近的文献表明，球形的替代形态可以提高检测限。在这项工作中，我们报告的应用替代金纳米粒子形态，金纳米带（长度约35 nm）和金纳米锥（直径约90 nm），在癌胚抗原的侧流检测。在比较测定中，对于相同的抗体负载和总金含量，与市售球形金纳米颗粒相比，金纳米锥表现出检测限的10.2倍提高，而每个测试中金纳米锥的数量少10.3.2倍。在完全优化的测试中，使用金纳米锥获得14.4 pg/mL的检测限，代表了比先前报道的基于金纳米颗粒的癌胚抗原侧流测定提高了24倍。
Colorectal cancer is the third most common malignancy and the second leading cause of cancer death globally. Multiple studies have linked levels of carcinoembryonic antigen in patient serum to poor disease prognosis. Hence, the ability to detect low levels of carcinoembryonic antigen has applications in earlier disease diagnosis, assessment, and recurrence monitoring. Existing carcinoembryonic antigen detection methods often require multiple reagents, trained operators, or complex procedures. A method alleviating these issues is the lateral flow assay, a paper-based platform that allows the detection and quantification of target analytes in complex mixtures. The tests are rapid, are point-of-care, possess a long shelf life, and can be stored at ambient conditions, making them ideal for use in a range of settings. Although lateral flow assays typically use spherical gold nanoparticles to generate the classic red signal, recent literature has shown that alternate morphologies to spheres can improve the limit of detection. In this work, we report the application of alternative gold nanoparticle morphologies, gold nanotapes (∼35 nm in length) and gold nanopinecones (∼90 nm in diameter), in a lateral flow assay for carcinoembryonic antigen. In a comparative assay, gold nanopinecones exhibited a ∼2× improvement in the limit of detection compared to commercially available spherical gold nanoparticles for the same antibody loading and total gold content, whereas the number of gold nanopinecones in each test was ∼3.2× less. In the fully optimized test, a limit of detection of 14.4 pg/mL was obtained using the gold nanopinecones, representing a 24-fold improvement over the previously reported gold-nanoparticle-based carcinoembryonic antigen lateral flow assay.
登录
查看更多内容
影响因子：
--
作者：
Sun Z;Wang F;Zhou Q;Yang S;Sun X;Wang G;Li Z;Zhang Z;Song J;Liu J;Yuan W
通讯作者：
Yuan W
影响因子：
6.4
作者：
Koczula KM;Gallotta A
通讯作者：
Gallotta A
影响因子：
3.7
作者：
Huang, Shu-Huan;Tsai, Wen-Sy;Chen, Jinn-Shiun
通讯作者：
Chen, Jinn-Shiun
影响因子：
8
作者：
Gong Y;Hu J;Choi JR;You M;Zheng Y;Xu B;Wen T;Xu F
通讯作者：
Xu F
影响因子：
7.4
作者：
Hendel, Thomas;Wuithschick, Maria;Polte, Joerg
通讯作者：
Polte, Joerg