Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila: A case of functional divergence in a multigene family.
Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila: A case of functional divergence in a multigene family.
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纤毛虫嗜热四膜虫 ATP 结合盒转运蛋白的全基因组鉴定和进化：多基因家族功能分歧的一个案例
DOI：
10.1186/1471-2148-10-330
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发表时间：
2010-10-27
影响因子：
3.4
通讯作者：
Miao W
中科院分区：
文献类型：
--
作者：
Xiong J;Feng L;Yuan D;Fu C;Miao W
背景在真核生物中，ABC转运蛋白利用ATP水解的能量将细胞底物排出到环境中，负责细胞的大部分外排。ABC转运蛋白超家族的许多成员与对多种药物或毒素的耐药性有关。由于其医疗和毒理学的重要性，ABC超家族的成员已被研究在几个模式生物和保证检查在新测序的genome.ResultsA共165 ABC转运蛋白基因，构成一个高度扩展的超家族相对于它的大小在其他真核生物中，被确定在大核基因组的纤毛虫四膜虫嗜热。根据同源性比较、系统发育拓扑学和内含子特征，将嗜热嗜热厌氧杆菌的ABC转运蛋白家族分为几个不同的类群，并对其进化关系提出了假设。一个全面的微阵列分析揭示了不同的表达模式之间的ABC转运蛋白超家族成员在不同的生理和发展状态。许多最近形成的重复对个别ABC转运蛋白家族表现出显着不同的表达模式。进一步的分析表明，多种机制导致了功能的分歧，是负责保存的duplicated genes.ConclusionGene duplications已导致ABC转运蛋白的超家族在theTetrahymenagenome的广泛扩展，使其成为迄今为止在任何生物体中报道的同类中最大的例子。多个独立的重复和随后的分歧促成了ABC转运蛋白基因不同家族的形成。基因家族中的许多成员表现出不同的表达模式。基因重复的组合，其次是序列的分歧和收购的新模式的表达可能发挥了作用，在适应ofTetrahymena它的环境。
BackgroundIn eukaryotes, ABC transporters that utilize the energy of ATP hydrolysis to expel cellular substrates into the environment are responsible for most of the efflux from cells. Many members of the superfamily of ABC transporters have been linked with resistance to multiple drugs or toxins. Owing to their medical and toxicological importance, members of the ABC superfamily have been studied in several model organisms and warrant examination in newly sequenced genomes.ResultsA total of 165 ABC transporter genes, constituting a highly expanded superfamily relative to its size in other eukaryotes, were identified in the macronuclear genome of the ciliateTetrahymena thermophila. Based on ortholog comparisons, phylogenetic topologies and intron characterizations, each highly expanded ABC transporter family ofT.thermophilawas classified into several distinct groups, and hypotheses about their evolutionary relationships are presented. A comprehensive microarray analysis revealed divergent expression patterns among the members of the ABC transporter superfamily during different states of physiology and development. Many of the relatively recently formed duplicate pairs within individual ABC transporter families exhibit significantly different expression patterns. Further analysis showed that multiple mechanisms have led to functional divergence that is responsible for the preservation of duplicated genes.ConclusionGene duplications have resulted in an extensive expansion of the superfamily of ABC transporters in theTetrahymenagenome, making it the largest example of its kind reported in any organism to date. Multiple independent duplications and subsequent divergence contributed to the formation of different families of ABC transporter genes. Many of the members within a gene family exhibit different expression patterns. The combination of gene duplication followed by both sequence divergence and acquisition of new patterns of expression likely plays a role in the adaptation ofTetrahymena to its environment.
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影响因子：
9.8
作者：
Conant GC;Wolfe KH
通讯作者：
Wolfe KH
影响因子：
--
作者：
Anjard, C;Loomis, WF
通讯作者：
Loomis, WF
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
2.9
作者：
Aurade, Ravindra;Jayalakshmi, Senigala K.;Sreeramulu, Kuruba
通讯作者：
Sreeramulu, Kuruba
纤毛虫嗜热四膜虫细胞色素 P450 单加氧酶基因的全基因组鉴定和表征
影响因子：
4.4
作者：
Fu C;Xiong J;Miao W
通讯作者：
Miao W