Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila.
Genome-wide identification and characterization of cytochrome P450 monooxygenase genes in the ciliate Tetrahymena thermophila.
复制标题
纤毛虫嗜热四膜虫细胞色素 P450 单加氧酶基因的全基因组鉴定和表征
DOI：
10.1186/1471-2164-10-208
复制
发表时间：
2009-05-01
期刊：
影响因子：
4.4
通讯作者：
Miao W
中科院分区：
文献类型：
--
作者：
Fu C;Xiong J;Miao W
背景
细胞色素P450单加氧酶在多种底物的代谢中起着关键作用，它们与细胞内的生理过程或环境暴露下的解毒代谢密切相关。然而，到目前为止，还没有一个在纤毛纲中有系统的特征。嗜热链霉菌作为真核模式生物具有许多优势，对外源生物具有快速、灵敏的反应，是研究P450单加氧酶基因家族进化和功能多样性的理想模型系统。
结果
共鉴定出44个可能具有功能的细胞色素P450基因，根据命名法可将其分为13个家族和21个亚家族。每个P450家族内保守的内含子-外显子组织和串联重复序列的骨架定位的特点表明，嗜热葡萄球菌P450家族的扩大可能是最近分离的小重复事件造成的。基于EST和基因芯片数据，分析了嗜热链霉菌P450在三个重要的细胞生理阶段(营养生长、饥饿和接合)的基因表达谱，推测了三类主要的表达谱。进化分析包括密码子使用偏好、位点特异性选择和基因表达进化模式，结果表明嗜热链霉菌P450基因之间存在显著差异。
结论
本研究中对嗜热嗜热菌P450单加氧酶基因的鉴定、表达和进化分析为了解纤毛虫P450基因的特征和多样性提供了有用的信息，并为该模式纤毛虫个体P450亚型的功能分析提供了基础。
Background
Cytochrome P450 monooxygenases play key roles in the metabolism of a wide variety of substrates and they are closely associated with endocellular physiological processes or detoxification metabolism under environmental exposure. To date, however, none has been systematically characterized in the phylum Ciliophora. T. thermophila possess many advantages as a eukaryotic model organism and it exhibits rapid and sensitive responses to xenobiotics, making it an ideal model system to study the evolutionary and functional diversity of the P450 monooxygenase gene family.
Results
A total of 44 putative functional cytochrome P450 genes were identified and could be classified into 13 families and 21 sub-families according to standard nomenclature. The characteristics of both the conserved intron-exon organization and scaffold localization of tandem repeats within each P450 family clade suggested that the enlargement of T. thermophila P450 families probably resulted from recent separate small duplication events. Gene expression patterns of all T. thermophila P450s during three important cell physiological stages (vegetative growth, starvation and conjugation) were analyzed based on EST and microarray data, and three main categories of expression patterns were postulated. Evolutionary analysis including codon usage preference, site-specific selection and gene-expression evolution patterns were investigated and the results indicated remarkable divergences among the T. thermophila P450 genes.
Conclusion
The characterization, expression and evolutionary analysis of T. thermophila P450 monooxygenase genes in the current study provides useful information for understanding the characteristics and diversities of the P450 genes in the Ciliophora, and provides the baseline for functional analyses of individual P450 isoforms in this model ciliate species.
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