Transcriptional network of the industrial hybrid Saccharomyces pastorianus reveals temperature-dependent allele expression bias and preferential orthologous protein assemblies
Transcriptional network of the industrial hybrid Saccharomyces pastorianus reveals temperature-dependent allele expression bias and preferential orthologous protein assemblies
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工业杂交巴斯德酵母的转录网络揭示了温度依赖性等位基因表达偏差和优先的直系同源蛋白质组装
DOI：
10.1101/2021.04.07.438844
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Timouma S
中科院分区：
文献类型：
--
作者：
Timouma S
巴斯德酵母是一种天然酵母，由不同的中温菌之间的杂交事件进化而来。以及耐寒植物。尤伯亚努斯这种复杂的非整倍体杂种携带多个拷贝的亲本等位基因以及特定的杂种基因，并编码多种蛋白质同种型，这些蛋白质同种型赋予新的表型，例如在低温下发酵的强能力。这些特征导致对底物的竞争性竞争和过多的生化活性，从而导致独特的细胞代谢。在这里，我们研究了温度变化过程中不同的正向等位基因在巴斯德酵母中的转录特征。我们确定了温度依赖性介质非依赖性基因，并表明35%的调控依赖于细胞外亮氨酸的摄取，这表明亮氨酸代谢和温度反应之间的相互作用。对亲本等位基因表达的分析表明，大多数基因在亲本等位基因中优先表达，S。在参与冷适应的基因中，Eubayanus样等位基因显著过量。功能上冗余的亲本等位基因的存在可能会影响在杂交体中建立的蛋白质复合物的性质，其中两个亲本等位基因是竞争的。我们的表达数据表明，大多数的蛋白质复合物中的混合物的调查很可能是完全嵌合或unisspecific和冗余是不鼓励的，一种情况下，很好地符合基因平衡假说。本研究首次对S. pastorianusand在表达水平上为其独特的工业性状提供了合理性。
Saccharomyces pastorianusis a natural yeast evolved from different hybridization events between the mesophilicS. cerevisiaeand the cold-tolerantS. eubayanus. This complex aneuploid hybrid carries multiple copies of the parental alleles alongside specific hybrid genes and encodes for multiple protein isoforms which impart novel phenotypes, such as the strong ability to ferment at low temperature. These characteristics lead to agonistic competition for substrates and a plethora of biochemical activities, resulting in a unique cellular metabolism. Here, we investigated the transcriptional signature of the different orthologous alleles inS. pastorianusduring temperature shifts. We identified temperature-dependent media-independent genes and showed that 35% has their regulation dependent on extracellular leucine uptake, suggesting an interplay between leucine metabolism and temperature response. The analysis of the expression of ortholog parental alleles unveiled that the majority of the genes expresses preferentially one parental allele over the other and thatS. eubayanus-like alleles are significantly over-represented among the genes involved in the cold acclimatization. The presence of functionally redundant parental alleles may impact on the nature of protein complexes established in the hybrid, where both parental alleles are competing. Our expression data indicate that the majority of the protein complexes investigated in the hybrid are likely to be either exclusively chimeric or unispecific and that the redundancy is discouraged, a scenario that fits well with the gene balance hypothesis. This study offers the first overview of the transcriptional pattern ofS. pastorianusand provides a rationalization for its unique industrial traits at the expression level.
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影响因子：
4.5
作者：
Timouma S;Schwartz JM;Delneri D
通讯作者：
Delneri D
影响因子：
2.6
作者：
Gibson, Brian R.;Storgards, Erna;Vidgren, Virve
通讯作者：
Vidgren, Virve
DOI：
10.1111/j.1469-8137.2009.03087.x
发表时间：
2010-04
期刊：
The New phytologist
影响因子：
--
作者：
Birchler JA;Veitia RA
通讯作者：
Veitia RA
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影响因子：
--
作者：
P. Rijken;B. Kruijff;A. D. de Kroon
通讯作者：
A. D. de Kroon
影响因子：
2.5
作者：
Bond, U;Neal, C;James, TC
通讯作者：
James, TC