Comparative morphology and transcriptome analysis reveals distinct functions of the primary and secondary laticifer cells in the rubber tree
Comparative morphology and transcriptome analysis reveals distinct functions of the primary and secondary laticifer cells in the rubber tree
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比较形态和转录组分析揭示了橡胶树初级和次级乳汁细胞的不同功能
DOI：
10.1038/s41598-017-03083-3
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发表时间：
2017-06
期刊：
影响因子：
--
通讯作者：
张家明
中科院分区：
文献类型：
--
作者：
谭德冠;胡小文;付莉莉;Anuwat Kumpeangkeaw;丁泽红;孙雪飘;张家明
橡胶树（Hevea brasiliensis Muell. Arg.）是一种多年生作物，在传统育种实践中，其育种周期约为30年。由于缺乏将幼树和成年树联系起来的合适参数，早期选择尝试效率低下。我们对幼枝诱导的愈伤组织中的乳管细胞以及成年树树干中的初生乳管和次生乳管进行了比较形态学研究，并探讨了它们与橡胶产量的关系。结果表明，次生乳管侧壁光滑，端壁有穿孔，属于有节的、连接成网的乳管。初生乳管无分支、无节且连接成网，成熟时侧壁有凸起。愈伤组织衍生的乳管在形态上与次生乳管相似，侧壁光滑且存在端壁，相对细胞壁厚度也相似。愈伤组织中的乳管密度和树皮中的乳管环数均依赖于基因型，并且它们都与橡胶产量高度相关，愈伤组织和树皮的相关系数分别为R = 0.846（P < 0.002）和R = 0.899（P < 0.002）。由于可以从异花授粉群体的幼苗组织诱导出愈伤组织，愈伤组织中的乳管密度可作为幼苗高产橡胶的早期选择标记，并可用于缩短橡胶树的育种周期。
The rubber tree (Hevea brasiliensis Muell. Arg.) is a perennial crop with a breeding cycle of approximately 30 years.in the conventional breeding practices. The lack of suitable parameters linking the juvenile and adult trees has made the early.selection attempts inefficient. We performed comparative morphology of laticifer cells in callus induced from young shoots.and the primary and secondary laticifers in the trunk of adult trees, and investigated their relationships to rubber yields..Results indicate that the secondary laticifers have smooth lateral walls and perforated end walls and belong to articulated.anastomosing laticifers. The primary laticifers are unbranched, non-articulated, and anastomosing, and have bumpy lateral.walls when matured. The callus-derived laticifers are morphologically similar to the secondary laticifers with smooth lateral.walls and the existence of end walls, and similar relative cell wall thickness. The laticifer density in callus and the laticifer.ring number in bark are both genotype-dependent, and they are both highly correlated with the rubber yields with correlation.coefficients R = 0.846 (P < 0.002) and R = 0.899 (P < 0.002) for the callus and bark, respectively. Since callus can be induced.from tissues of young seedlings in cross-pollinated populations, the laticifer density in callus can serve as an early selection.marker of seedlings for high rubber yields and may be used to shorten the rubber tree breeding cycle.
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影响因子：
2.9
作者：
Fudali, Sylwia;Sobczak, Miroslaw;Golinowski, Wladyslaw
通讯作者：
Golinowski, Wladyslaw
影响因子：
12.3
作者：
Robinson MD;Oshlack A
通讯作者：
Oshlack A
影响因子：
3.5
作者：
Lechward, Katarzyna;Sugajska, Ewa;Zolnierowicz, Stanislaw
通讯作者：
Zolnierowicz, Stanislaw
影响因子：
1.1
作者：
A. Funk
通讯作者：
A. Funk
影响因子：
3.7
作者：
Kour A;Boone AM;Vodkin LO
通讯作者：
Vodkin LO