RNA-Seq profiling of a defective seed coat mutation in Glycine max reveals differential expression of proline-rich and other cell wall protein transcripts.
RNA-Seq profiling of a defective seed coat mutation in Glycine max reveals differential expression of proline-rich and other cell wall protein transcripts.
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DOI：
10.1371/journal.pone.0096342
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Vodkin LO
中科院分区：
文献类型：
--
作者：
Kour A;Boone AM;Vodkin LO
植物细胞壁执行许多基本功能，包括为许多不同的细胞类型提供形状，并作为对潜在病原体的防御。由于细胞壁破裂，净模式突变在大豆（Glycine max）的种皮中产生断裂。使用RNA-Seq，我们检查了来自未成熟种子发育的三个阶段的种皮转录组，这两对具有正常或有缺陷的种皮表型的等值线，由于净模式。全基因组的比较研究，这些等值线的转录谱揭示了364差异表达的基因在这两个品种之间的共同之处，进一步分为不同的广泛的功能类别。在100-200 mg粒重的发育中期，与细胞壁突起相关的基因占差异表达基因的19%。在这一类中，细胞壁富含脯氨酸和富含甘氨酸的蛋白质基因在两种遗传背景中高度差异表达。在100-200 mg种子重量阶段在每个等值线对中显示显著表达变化的其他基因是木质部丝氨酸蛋白酶、成束蛋白相关基因、生长素和胁迫响应相关基因、透明种皮1（TT 1）和其他转录因子。突变体似乎改变了一些转录物水平增加或减少的时间。对这些数据集的分析揭示了种皮在细胞壁破裂形成期间所经历的生理变化。
The plant cell wall performs a number of essential functions including providing shape to many different cell types and serving as a defense against potential pathogens. The net pattern mutation creates breaks in the seed coat of soybean (Glycine max) because of ruptured cell walls. Using RNA-Seq, we examined the seed coat transcriptome from three stages of immature seed development in two pairs of isolines with normal or defective seed coat phenotypes due to the net pattern. The genome-wide comparative study of the transcript profiles of these isolines revealed 364 differentially expressed genes in common between the two varieties that were further divided into different broad functional categories. Genes related to cell wall processes accounted for 19% of the differentially expressed genes in the middle developmental stage of 100–200 mg seed weight. Within this class, the cell wall proline-rich and glycine-rich protein genes were highly differentially expressed in both genetic backgrounds. Other genes that showed significant expression changes in each of the isoline pairs at the 100–200 mg seed weight stage were xylem serine proteinase, fasciclin-related genes, auxin and stress response related genes, TRANSPARENT TESTA 1 (TT1) and other transcription factors. The mutant appears to shift the timing of either the increase or decrease in the levels of some of the transcripts. The analysis of these data sets reveals the physiological changes that the seed coat undergoes during the formation of the breaks in the cell wall.
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影响因子：
5.3
作者：
Gillman JD;Tetlow A;Lee JD;Shannon JG;Bilyeu K
通讯作者：
Bilyeu K
影响因子：
14.9
作者：
Grant D;Nelson RT;Cannon SB;Shoemaker RC
通讯作者：
Shoemaker RC
DOI：
10.1073/pnas.94.12.6559
发表时间：
1997-06-10
影响因子：
11.1
作者：
Cosgrove, DJ;Bedinger, P;Durachko, DM
通讯作者：
Durachko, DM
影响因子：
7.1
作者：
BAILEY, RW;CHESSON, A;MONRO, J
通讯作者：
MONRO, J
影响因子：
14.9
作者：
Goodstein DM;Shu S;Howson R;Neupane R;Hayes RD;Fazo J;Mitros T;Dirks W;Hellsten U;Putnam N;Rokhsar DS
通讯作者：
Rokhsar DS