The effect of terminal sterilization on structural and biophysical properties of a decellularized collagen-based scaffold; implications for stem cell adhesion.
The effect of terminal sterilization on structural and biophysical properties of a decellularized collagen-based scaffold; implications for stem cell adhesion.
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DOI：
10.1002/jbm.b.33213
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发表时间：
2015-02
影响因子：
3.4
通讯作者：
McFetridge, Peter S.
中科院分区：
文献类型：
--
作者：
Matuska, Andrea M.;McFetridge, Peter S.
终末灭菌诱导体外衍生生物材料的细胞外基质（ECM）发生物理和化学变化，这是由于其具有攻击性的作用机制。之前的研究主要集中在灭菌如何影响基于组织的生物材料的机械完整性，但很少表征对早期细胞相互作用的影响，这表明了生物反应。使用模型纤维软骨盘支架，这些研究比较了三种常见的灭菌方法[过乙酸（PAA），伽马辐照（GI）和环氧乙烷（EtO）]对一系列材料特性和早期细胞相互作用的影响。GI和EtO产生了不利的结构损伤，导致细胞粘附性较差。相反，暴露于PAA导致有限的结构改变，同时诱导有利于细胞附着的化学修饰。结果表明，可以选择灭菌方法来调节生物材料特性，以有利于细胞粘附，并在组织工程和再生医学应用中具有相关性。此外，体外细胞与改性生物材料相互作用的研究提供了材料在后续临床应用中如何反应的信息。
Terminal sterilization induces physical and chemical changes in the extracellular matrix (ECM) of ex vivo-derived biomaterials due to their aggressive mechanism of action. Prior studies have focused on how sterilization affects the mechanical integrity of tissue-based biomaterials but have rarely characterized effects on early cellular interaction, which is indicative of the biological response. Using a model fibro-cartilage disc scaffold, these investigations compare the effect of three common sterilization methods [peracetic acid (PAA), gamma irradiation (GI), and ethylene oxide (EtO)] on a range of material properties and characterized early cellular interactions. GI and EtO produced unfavorable structural damage that contributed to inferior cell adhesion. Conversely, exposure to PAA resulted in limited structural alterations while inducing chemical modifications that favored cell attachment. Results suggest that the sterilization approach can be selected to modulate biomaterial properties to favor cellular adhesion and has relevance in tissue engineering and regenerative medicine applications. Furthermore, the study of cellular interactions with modified biomaterials in vitro provides information of how materials may react in subsequent clinical applications.
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影响因子：
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作者：
Bailey, Mark M.;Wang, Limin;Detamore, Michael S.
通讯作者：
Detamore, Michael S.
影响因子：
1.7
作者：
Haimi, Suvi;Vienonen, Annika;Suuronen, Riitta
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14
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影响因子：
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作者：
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通讯作者：
FOWKES, FM
影响因子：
4.8
作者：
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通讯作者：
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