Viscosity-difference-induced asymmetric selective focusing for large stroke particle separation
Viscosity-difference-induced asymmetric selective focusing for large stroke particle separation
复制标题
用于大冲程颗粒分离的粘度差引起的不对称选择性聚焦
DOI：
10.1007/s10404-016-1791-5
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发表时间：
2016-08
影响因子：
2.8
通讯作者：
Zhigang Wu
中科院分区：
文献类型：
--
作者：
Wenchao Xu;Zining Hou;Zhenhua Liu;Zhigang Wu
提出了一种新的粒子分离方法，通过引入鞘流的粘性差，形成样品粒子流的非对称聚焦。该方法依赖于颗粒流的非对称聚焦中的高速梯度来产生升力，该升力在颗粒分离中起主导作用。较大的颗粒从原始流线向相对速度较高的一侧迁移，而较小的颗粒保持接近流线。在高粘度（甘油-水溶液）和低粘度（PBS）鞘流下，在样品微流体装置中实现了较小（1.0 μ m）和较大（9.9 μ m）颗粒之间的显著大冲程分离。我们证明，鞘流的流速和粘度差对颗粒分离的间隔距离有影响，该间隔距离影响收集的纯度，并且对影响收集的浓度的较小颗粒的聚焦分布有影响。在1042 μ m宽的器件分离区中，获得了293 μ m的间隔距离（相对于沟道宽度：0.281）和112 μ m的聚焦分布（相对于沟道宽度：0.107）。在我们的工作中提出的这种分离方法可以潜在地应用于生物和医学应用，由于宽的间隔距离和窄的颗粒分离的聚焦分布，通过在简单的设备中容易地制造。
We developed a new approach for particle separation by introducing viscosity difference of the sheath flows to form an asymmetric focusing of sample particle flow. This approach relies on the high-velocity gradient in the asymmetric focusing of the particle flow to generate a lift force, which plays a dominated role in the particle separation. The larger particles migrate away from the original streamline to the side of the higher relative velocity, while the smaller particles remain close to the streamline. Under high-viscosity (glycerol-water solution) and low-viscosity (PBS) sheath flows, a significant large stroke separation between the smaller (1.0 mu m) and larger (9.9 mu m) particles was achieved in a sample microfluidic device. We demonstrate that the flow rate and the viscosity difference of the sheath flows have an impact on the interval distance of the particle separation that affects the collected purity and on the focusing distribution of the smaller particles that affects the collected concentration. The interval distance of 293 mu m (relative to the channel width: 0.281) and the focusing distribution of 112 mu m (relative to the channel width: 0.107) were obtained in the 1042-mu m-width separation area of the device. This separation method proposed in our work can potentially be applied to biological and medical applications due to the wide interval distance and the narrow focusing distribution of the particle separation, by easy manufacturing in a simple device.
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影响因子：
6.1
作者：
Huang, Song-Bin;Wu, Min-Hsien;Lee, Gwo-Bin
通讯作者：
Lee, Gwo-Bin
影响因子：
3.2
作者：
Jian Zhou;P. Giridhar;S. Kasper;I. Papautsky
通讯作者：
Jian Zhou;P. Giridhar;S. Kasper;I. Papautsky
影响因子：
6.1
作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
6.1
作者：
Wu, Zhigang;Willing, Ben;Hjort, Klas
通讯作者：
Hjort, Klas
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC