Does Impedance Matter When Recording Spikes With Polytrodes?
Does Impedance Matter When Recording Spikes With Polytrodes?
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DOI：
10.3389/fnins.2018.00715
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发表时间：
2018
影响因子：
4.3
通讯作者：
Kampff AR
中科院分区：
文献类型：
--
作者：
Neto JP;Baião P;Lopes G;Frazão J;Nogueira J;Fortunato E;Barquinha P;Kampff AR
细胞外微电极已被广泛用于测量大脑活动，但仍有一个良好的细胞外微电极的要求的基本问题。一个常见的混淆来源是电极的阻抗对细胞外尖峰和背景噪声的幅度有多大影响。在这里，我们量化了电极阻抗对细胞外记录数据质量的影响，这对于检测尖峰信号及其分配给正确的神经元至关重要。本研究采用了包含32个电极（177 μm2）的商用多极电极，这些电极以密集阵列排列。这使我们能够直接比较，并排，相同的细胞外信号测量的修改后的低阻抗（100 kΩ）微电极与未修改的高阻抗（101 MΩ）微电极。我们开始评估现有的协议，以降低电极的阻抗。聚（3，4-乙撑二氧噻吩）-聚苯乙烯磺酸盐（PEDOT-PSS）电沉积方案是一种简单，稳定，可靠的方法，降低微电极的阻抗高达10倍。接下来，我们使用以“棋盘”图案修饰的多电极进行体内记录，使得一个神经元的信号被多个涂层和非涂层电极检测到。然后在背景噪声和检测到的动作电位的幅度的测量上比较涂覆和未涂覆电极的性能。如果使用适当的记录系统，则在标准多电极范围内的微电极阻抗（10.1至2 MΩ）不会对数据质量和尖峰分选产生很大影响。这项研究应该鼓励神经科学家停止担心另一个未知数。
Extracellular microelectrodes have been widely used to measure brain activity, yet there are still basic questions about the requirements for a good extracellular microelectrode. One common source of confusion is how much an electrode’s impedance affects the amplitude of extracellular spikes and background noise. Here we quantify the effect of an electrode’s impedance on data quality in extracellular recordings, which is crucial for both the detection of spikes and their assignment to the correct neurons. This study employs commercial polytrodes containing 32 electrodes (177 μm2) arranged in a dense array. This allowed us to directly compare, side-by-side, the same extracellular signals measured by modified low impedance (∼100 kΩ) microelectrodes with unmodified high impedance (∼1 MΩ) microelectrodes. We begin with an evaluation of existing protocols to lower the impedance of the electrodes. The poly (3,4-ethylenedioxythiophene)-polystyrene sulfonate (PEDOT-PSS) electrodeposition protocol is a simple, stable, and reliable method for decreasing the impedance of a microelectrode up to 10-fold. We next record in vivo using polytrodes that are modified in a ‘chess board’ pattern, such that the signal of one neuron is detected by multiple coated and non-coated electrodes. The performance of the coated and non-coated electrodes is then compared on measures of background noise and amplitude of the detected action potentials. If the proper recording system is used, then the impedance of a microelectrode within the range of standard polytrodes (∼0.1 to 2 MΩ) does not greatly affect data quality and spike sorting. This study should encourage neuroscientists to stop worrying about one more unknown.
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DOI：
10.3389/fneng.2010.00005
发表时间：
2010
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
Desai SA;Rolston JD;Guo L;Potter SM
通讯作者：
Potter SM
影响因子：
4.3
作者：
Obien ME;Deligkaris K;Bullmann T;Bakkum DJ;Frey U
通讯作者：
Frey U
使用纳米制造的神经探针进行多重、高密度电生理学。
影响因子：
3.7
作者：
Du J;Blanche TJ;Harrison RR;Lester HA;Masmanidis SC
通讯作者：
Masmanidis SC
影响因子：
14
作者：
Green, Rylie A.;Lovell, Nigel H.;Poole-Warren, Laura A.
通讯作者：
Poole-Warren, Laura A.
影响因子：
4.6
作者：
Cui, XY;Martin, DC
通讯作者：
Martin, DC