Preserving human cells for regenerative, reproductive, and transfusion medicine.

Preserving human cells for regenerative, reproductive, and transfusion medicine.
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DOI:
10.1002/biot.201300074
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发表时间:
2014-07
影响因子:
4.7
通讯作者:
Demirci, Utkan
Demirci, Utkan
中科院分区:
工程技术2区
文献类型:
--
作者:
Asghar, Waseem;El Assal, Rami;Shafiee, Hadi;Anchan, Raymond M.;Demirci, Utkan

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细胞冷冻保存通过减缓生物化学过程使细胞在零度以下的温度下保持生命。在现代生殖、再生和输血医学中,各种类型的细胞被常规地冷冻保存。目前的细胞冷冻保存方法涉及在冷冻保护剂(CPA)存在下冷冻(缓慢/快速)或玻璃化细胞。尽管这些方法在临床上使用,但由于冰晶、渗透压休克和CPA毒性引起的冷冻损伤导致细胞活力和功能丧失。最近的方法,使用最小体积的玻璃化提供了替代传统的冷冻保存方法。最小体积玻璃化提供了超高的冷却和复温速率,使得能够保存细胞而不形成冰晶。在此,我们回顾了细胞冷冻保存技术的最新进展,并提供了卵母细胞,干细胞和红细胞冷冻保存技术的例子。
Cell cryopreservation enables maintaining cellular life at sub-zero temperatures by slowing down biochemical processes. Various cell types are routinely cryopreserved in modern reproductive, regenerative, and transfusion medicine. Current cell cryopreservation methods involve freezing (slow/rapid) or vitrifying cells in the presence of a cryoprotective agent (CPA). Although these methods are clinically utilized, cryo-injury due to ice crystals, osmotic shock, and CPA toxicity cause loss of cell viability and function. Recent approaches using minimum volume vitrification provide alternatives to the conventional cryopreservation methods. Minimum volume vitrification provides ultra-high cooling and rewarming rates that enable preserving cells without ice crystal formation. Herein, we review recent advances in cell cryopreservation technology and provide examples of techniques that are utilized in oocyte, stem cell, and red blood cell cryopreservation.
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