Advances in microfluidic cell separation and manipulation.

Advances in microfluidic cell separation and manipulation.
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微流体细胞分离和操纵的进展。

DOI:
10.1016/j.coche.2013.10.001
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发表时间:
2013-11-01
影响因子:
6.6
通讯作者:
Lu H
Lu H
中科院分区:
工程技术2区
文献类型:
--
作者:
Jackson EL;Lu H

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在生物化学和生物医学应用的许多情况下,需要细胞分离来鉴定、分离和分析感兴趣的表型或样品。微流体技术独特地适合处理生物样本,新兴技术已成为研究人员和临床医生越来越容易使用的工具。在这里,我们回顾了在过去几年中的微流控细胞分离和操纵技术的进展。高通量细胞和生物体表型筛选、异质干细胞群纯化、血液成分分离和患者稀有细胞分离等应用突出了这些技术显示出巨大潜力的一些领域。在分离机制和细胞系统的理解方面的持续进步将进一步提高技术的通量、分辨率和鲁棒性。
Cellular separations are required in many contexts in biochemical and biomedical applications for the identification, isolation, and analysis of phenotypes or samples of interest. Microfluidics is uniquely suited for handling biological samples, and emerging technologies have become increasingly accessible tools for researchers and clinicians. Here, we review advances in the last few years in techniques for microfluidic cell separation and manipulation. Applications such as high-throughput cell and organism phenotypic screening, purification of heterogeneous stem cell populations, separation of blood components, and isolation of rare cells in patients highlight some of the areas in which these technologies show great potential. Continued advances in separation mechanisms and understanding of cellular systems will yield further improvements in the throughput, resolution, and robustness of techniques.
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发表时间: 2010-06-21
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影响因子: 6.1
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