Bacteria-Derived Hemolysis-Related Genes Widely Exist in Scuticociliates.
Bacteria-Derived Hemolysis-Related Genes Widely Exist in Scuticociliates.
复制标题
细菌源性溶血相关基因广泛存在于盾纤毛虫中。
DOI：
10.3390/microorganisms8111838
复制
发表时间：
2020-11-22
期刊：
影响因子：
4.5
通讯作者：
Xiong J
中科院分区：
文献类型：
--
作者：
Zhang J;Chen K;Jiang C;Yang W;Gu S;Wang G;Lu Y;Miao W;Xiong J
盾纤毛病是由纤毛原虫引起的侵入性外部或全身感染，主要是盾纤毛亚纲（盾纤毛虫）内的原虫。许多盾纤毛虫是鱼类病原体，包括amiamiensis avidus、philasterides dicentrarchi、Pseudocohnilembus persalinus 和Uronema marinum。我们之前的研究表明，通过水平基因转移（HGT）从细菌中获得的溶血相关基因可能有助于 P. persalinus 的毒力。出血性病变是盾纤毛虫病的共同特征，但尚不清楚其他盾纤毛虫是否也具有细菌源性溶血相关基因。在这项研究中，我们构建了另一种典型致病性盾纤毛虫——海藻的高质量大核基因组。在该物种中总共鉴定出105个HGT基因，其中35个是先前在P. persalinus中鉴定的溶血相关基因（包括溶血素样基因）的同源物。对来自四个盾纤毛虫科的另外五个物种的测序表明，细菌来源的溶血相关基因（特别是溶血素样基因）广泛分布于盾纤毛虫中。基于这些发现，我们认为溶血素样基因可能起源于盾纤毛虫分化之前。
Scuticociliatosis is an invasive external or systemic infection caused by ciliated protozoa, mainly those within the subclass Scuticociliatia (scuticociliates). Many scuticociliates are fish pathogens, including Miamiensis avidus, Philasterides dicentrarchi, Pseudocohnilembus persalinus, and Uronema marinum. Our previous study showed that hemolysis-related genes derived from bacteria through horizontal gene transfer (HGT) may contribute to virulence in P. persalinus. Hemorrhagic lesions are a common feature of scuticociliatosis, but it is not known whether other scuticociliates also have bacteria-derived hemolysis-related genes. In this study, we constructed a high-quality macronuclear genome of another typical pathogenic scuticociliate, U. marinum. A total of 105 HGT genes were identified in this species, of which 35 were homologs of hemolysis-related genes (including hemolysin-like genes) that had previously been identified in P. persalinus. Sequencing of an additional five species from four scuticociliate families showed that bacteria-derived hemolysis-related genes (especially hemolysin-like genes) are widely distributed in scuticociliates. Based on these findings, we suggest that hemolysin-like genes may have originated before the divergence of scuticociliates.
登录
查看更多内容
系统发育分析表明，Scuticociliatia 亚纲是 Oligohymenophorea 纲 Hymenostomatia 亚纲的姐妹群
影响因子：
4.1
作者：
Feng, Jin-Mei;Jiang, Chuan-Qi;Miao, Wei
通讯作者：
Miao, Wei
影响因子：
64.8
作者：
Aury, Jean-Marc;Jaillon, Olivier;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
4.1
作者：
Gao, Feng;Katz, Laura A.;Song, Weibo
通讯作者：
Song, Weibo
影响因子：
3.4
作者：
Baida, GE;Kuzmin, NP
通讯作者：
Kuzmin, NP
DOI：
10.1002/dvg.1020130206
发表时间：
1992-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
DOERDER, FP;DEAK, JC;LIEF, JH
通讯作者：
LIEF, JH