A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance.
A secretory protein of necrotrophic fungus Sclerotinia sclerotiorum that suppresses host resistance.
复制标题
抑制宿主抗性的坏死性真菌核盘菌的分泌蛋白
DOI：
10.1371/journal.pone.0053901
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Jiang D
中科院分区：
文献类型：
--
作者：
Zhu W;Wei W;Fu Y;Cheng J;Xie J;Li G;Yi X;Kang Z;Dickman MB;Jiang D
核盘菌（Sclerotinia sclerotiorum）SSITL（SS1G_14133）编码一个含信号肽的302个氨基酸残基的蛋白质，通过免疫定位和免疫荧光技术证实了其分泌特性。SSITL属于整合素α N-末端结构域超家族，其三维结构与人整合素α4亚基和真菌整合素样蛋白的三维结构相似。当S.接种到寄主上后，SSITL在感染的初期（1.5-3.0 hpi）有较高的表达。SSITL基因的靶向沉默可显著降低拟南芥的毒力，而SSITL基因的沉默可诱导拟南芥产生强烈而快速的防御反应，防御基因PDF 1. 2和PR-1在接种后3 h达到最高表达，比野生型S. p.菌核。通过分析PDF1.2和PR-1的表达来检测A10诱导的系统抗性，并在接种灰葡萄孢后证实。与野生型植物相比，A10在拟南芥突变体ein 2和jar 1上诱导了大得多的病变，在突变体pad 4和NahG上诱导了稍大的病变。此外，SSITL在拟南芥中的瞬时表达和组成型表达均抑制了PDF1.2的表达，并导致对A10和野生型S. sclerotiorum和B.灰叶这些结果表明，SSITL可能是一种效应子，在侵染早期抑制茉莉酸/乙烯（JA/ET）信号通路介导的抗病性中起重要作用。
SSITL (SS1G_14133) of Sclerotinia sclerotiorum encodes a protein with 302 amino acid residues including a signal peptide, its secretion property was confirmed with immunolocalization and immunofluorescence techniques. SSITL was classified in the integrin alpha N-terminal domain superfamily, and its 3D structure is similar to those of human integrin α4-subunit and a fungal integrin-like protein. When S. sclerotiorum was inoculated to its host, high expression of SSITL was detected during the initial stages of infection (1.5–3.0 hpi). Targeted silencing of SSITL resulted in a significant reduction in virulence; on the other hand, inoculation of SSITL silenced transformant A10 initiated strong and rapid defense response in Arabidopsis, the highest expressions of defense genes PDF1.2 and PR-1 appeared at 3 hpi which was 9 hr earlier than that time when plants were inoculated with the wild-type strain of S. sclerotiorum. Systemic resistance induced by A10 was detected by analysis of the expression of PDF1.2 and PR-1, and confirmed following inoculation with Botrytis cinerea. A10 induced much larger lesions on Arabidopsis mutant ein2 and jar1, and slightly larger lesions on mutant pad4 and NahG in comparison with the wild-type plants. Furthermore, both transient and constitutive expression of SSITL in Arabidopsis suppressed the expression of PDF1.2 and led to be more susceptible to A10 and the wild-type strain of S. sclerotiorum and B. cinerea. Our results suggested that SSITL is an effector possibly and plays significant role in the suppression of jasmonic/ethylene (JA/ET) signal pathway mediated resistance at the early stage of infection.
登录
查看更多内容
影响因子：
3.5
作者：
Guo, Xiaomei;Stotz, Henrik U.
通讯作者：
Stotz, Henrik U.
影响因子：
7.2
作者：
Ferrari, S;Plotnikova, JM;Ausubel, FM
通讯作者：
Ausubel, FM
影响因子：
56.9
作者：
Gale, CA;Bendel, CM;Hostetter, MK
通讯作者：
Hostetter, MK
影响因子：
11.4
作者：
Dai, FM;Xu, T;He, ZH
通讯作者：
He, ZH
影响因子：
11.6
作者：
Cessna, SG;Sears, VE;Low, PS
通讯作者：
Low, PS