The expression of HLA antigens by cells in the human cornea.

The expression of HLA antigens by cells in the human cornea.
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人角膜细胞表达 HLA 抗原。

DOI:
10.1016/0002-9394(84)90696-2
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发表时间:
1984
影响因子:
4.2
通讯作者:
Sanfilippo,F
Sanfilippo,F
中科院分区:
医学1区
文献类型:
--
作者:
Treseler,PA;Foulks,GN;Sanfilippo,F

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尽管人类主要组织相容性复合体HLA的抗原似乎参与角膜同种异体移植物的排斥反应,但人类角膜细胞对这些抗原的表达仍存在争议。使用敏感的免疫过氧化物酶技术,我们很容易证明I类HLA抗原的角膜上皮细胞,基质细胞和内皮细胞,无论供体年龄。此外,I类抗原的上皮细胞的表达从中央到周边角膜显着增加。最后,发现II类HLA抗原的细胞分散在整个中央和外周上皮以及基质。从已知HLA类型的供体获得的角膜在角膜中表达所有预期的且没有意外的多态性HLA决定簇,其模式与单态性HLA决定簇相似。
Although antigens of the human major histocompatibility complex, HLA, appear to be involved in the rejection of corneal allografts, the expression of these antigens by cells of the human cornea remains controversial. Using sensitive immunoperoxidase techniques, we readily demonstrated class I HLA antigens on corneal epithelial, stromal, and endothelial cells, regardless of donor age. Moreover, the expression of class I antigens by epithelium increased markedly from the central to the peripheral cornea. Finally, class II HLA antigens were found on cells scattered throughout both central and peripheral epithelium as well as the stroma. A cornea obtained from a donor of known HLA type expressed all expected and no unexpected polymorphic HLA determinants in the cornea, in a pattern similar to that seen for monomorphic HLA determinants.
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