Creation of bony microenvironment with CaP and cell-derived ECM to enhance human bone-marrow MSC behavior and delivery of BMP-2.
Creation of bony microenvironment with CaP and cell-derived ECM to enhance human bone-marrow MSC behavior and delivery of BMP-2.
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DOI：
10.1016/j.biomaterials.2011.05.015
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发表时间：
2011-09
期刊：
影响因子：
14
通讯作者：
Yang, Yunzhi
中科院分区：
文献类型：
--
作者：
Kang, Yunqing;Kim, Sungwoo;Khademhosseini, Ali;Yang, Yunzhi
关键词：
细胞外基质（Extracellular matrix， ECM）由丰富的蛋白质和蛋白聚糖组成，不仅包含细胞行为的生物学线索，而且是结合生长因子并控制其释放的储存库。在这里，我们的目标是用细胞来源的ECM和可生物降解的β-磷酸三钙（β-TCP）创造一个合适的骨微环境。更具体地说，我们研究了由骨髓间充质干细胞（hBMSC）在β-TCP支架上产生的ECM是否可以结合骨形态发生蛋白-2 （BMP-2）并持续控制其释放，并进一步研究了ECM和ECM释放的BMP-2对细胞行为的影响。ECM是通过在β-TCP多孔支架上培养hBMSC并进行脱细胞和灭菌获得的。SEM、XPS、FTIR和免疫荧光染色结果显示，β-TCP上存在ECM，且ECM的数量随孵育时间的延长而增加。通过将BMP-2浸泡在BMP-2溶液中，将BMP-2加载到有ECM和没有ECM的β-TCP上。BMP-2在β-TCP/ECM上的加载和释放动力学明显慢于β-TCP。β-TCP/ECM表现出BMP-2的缓释特征，这也受ECM量的影响。这可能是因为β-TCP/ECM与BMP-2的结合机制不同。β-TCP/ECM促进细胞增殖。此外，与未加载BMP-2和加载BMP-2的β-TCP相比，加载BMP-2的β-TCP/ECM刺激了肌动蛋白细胞骨架的重组，增加了碱性磷酸酶的表达和细胞钙沉积。
Extracellular matrix (ECM) comprises a rich meshwork of proteins and proteoglycans, which not only contains biological cues for cell behavior, but is also a reservoir for binding growth factors and controlling their release. Here we aimed to create a suitable bony microenvironment with cell-derived ECM and biodegradable β-tricalcium phosphate (β-TCP). More specifically, we investigated whether the ECM produced by bone marrow-derived mesenchymal stem cells (hBMSC) on a β-TCP scaffold can bind bone morphogenetic protein-2 (BMP-2) and control its release in a sustained manner, and further examined the effect of ECM and the BMP-2 released from ECM on cell behaviors. The ECM was obtained through culturing the hBMSC on a β-TCP porous scaffold and performing decellularization and sterilization. SEM, XPS, FTIR, and immunofluorescent staining results indicated the presence of ECM on the β-TCP and the amount of ECM increased with the incubation time. BMP-2 was loaded onto the β-TCP with and without ECM by immersing the scaffolds in the BMP-2 solution. The loading and release kinetics of the BMP-2 on the β-TCP/ECM were significantly slower than those on the β-TCP. The β-TCP/ECM exhibited a sustained release profile of the BMP-2, which was also affected by the amount of ECM. This is probably because the β-TCP/ECM has different binding mechanisms with BMP-2. The β-TCP/ECM promoted cell proliferation. Furthermore, the BMP-2-loaded β-TCP/ECM stimulated reorganization of the actin cytoskeleton, increased expression of alkaline phosphatase and calcium deposition by the cells compared to those without BMP-2 loading and the β-TCP with BMP-2 loading.
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影响因子：
14
作者：
Flynn, Lauren E.;Prestwich, Glenn D.;Woodhouse, Kimberly A.
通讯作者：
Woodhouse, Kimberly A.
DOI：
10.1016/j.msec.2009.04.013
发表时间：
2009-08-31
影响因子：
7.9
作者：
Coelho, Paulo G.;Coimbra, Maria E.;Marincola, Mauro
通讯作者：
Marincola, Mauro
影响因子：
6.2
作者：
Cho, TJ;Gerstenfeld, LC;Einhorn, TA
通讯作者：
Einhorn, TA
影响因子：
14
作者：
Jiang, Tao;Abdel-Fattah, Wafa I.;Laurencin, Cato T.
通讯作者：
Laurencin, Cato T.
DOI：
10.1073/pnas.0505661103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Datta, N;Pham, QP;Mikos, AG
通讯作者：
Mikos, AG