Neurotransmitters and Receptors Changes in Medial Nucleus of the Trapezoid Body (MNTB) of Early-Developmental Rats with Single-Side Deafness.
Neurotransmitters and Receptors Changes in Medial Nucleus of the Trapezoid Body (MNTB) of Early-Developmental Rats with Single-Side Deafness.
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单侧耳聋早期发育大鼠梯形体内侧核（MNTB）神经递质和受体的变化
DOI：
10.12659/msm.908432
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发表时间：
2018-01-20
期刊：
影响因子：
--
通讯作者：
Wang N
中科院分区：
文献类型：
--
作者：
Dai J;Liu J;Zhou M;Wang W;Xu ZD;Wang N
先天性单侧耳聋(SSD)在某些情况下甚至会影响人工耳蜗植入(CI)后的声音定位。斜方体内侧核(MNTB)在双耳受益和声音定位中起着重要作用，但对MNTB合并SSD的内在分子变化知之甚少。本实验旨在观察发育早期SSD大鼠MNTB的变化，包括关键神经递质(GABA、Gly、Glu)和主要受体(GABAA-R/GABAB-R为GABA，Gly-R为Gly-R，AMPA/NMDA为Glu)。在P12时，通过右侧耳蜗术获得早期发育的SSD模型，并经ABR证实。高效液相色谱荧光检测法测定MNTB内神经递质水平。用实时定量聚合酶链式反应检测神经递质受体的相对表达。(1)实验大鼠右侧MNTB中GABA、Gly和Glu含量在右侧耳蜗术后4周显著升高(P<0.05)。(2)实验2周时，实验大鼠左侧MNTB中GABAA-R、GABAB-R、Gly-R和AMPA表达增加，右侧MNTB中NMDA表达降低(P<0.05)。左侧MNTB的高级受体下降到GABAA-R和GABAB-R在1周时无差异的水平(P>；0.05)，Gly-R和AMPA甚至相反(P<；0.05)。(3)双侧MNTB左侧Gly水平在2周时显著升高，并持续至4周(P<；0.05)。早期发育的SSD可导致MNTB内神经递质和受体的不对称分布，这可能是人工耳蜗术后声音定位缺陷的根本原因。
Congenital single-side deafness (SSD) affects sound localization even after cochlear implantation (CI) in some conditions. The medial nucleus of the trapezoid body (MNTB) plays an important role in binaural benefit and sound localization, but little is known about intrinsic molecular changes in MNTB with SSD. We aimed to observe changes in MNTB in early-developmental SSD rats, including the key neurotransmitters (GABA, Gly, Glu) and major receptors (GABAa-R/GABAb-R for GABA, Gly-R for Gly, and AMPA/NMDA for Glu). The model of early-developmental SSD was acquired by right cochlear ablation at P12 and confirmed by ABR. High-performance liquid chromatography fluorescence detection (HPLC-FLD) was performed to measure the levels of neurotransmitters in MNTB. The relative expression of neurotransmitter receptors was tested by quantitative real-time PCR analysis. (1) The right MNTB of experimental rats had an increase in GABA, Gly, and Glu at 4 weeks after right cochlear ablation (P<0.05). (2) At 2 weeks, the left MNTB of experimental rats showed increases in GABAa-R, GABAb-R, Gly-R, and AMPA, while the right MNTB showed lower expression of NMDA (P<0.05). The higher receptors in left MNTB decreased to a level at which we found no difference at 1 week for GABAa-R and GABAb-R (P>0.05), and was even reversed for Gly-R and AMPA (P<0.05). (3) Gly level was significantly increased at 2 weeks bilaterally and continued to 4 weeks in the left MNTB (P<0.05). Early-developmental SSD can lead to asymmetric distribution of neurotransmitters and receptors in MNTB, which can be the fundamental cause of defective sound localization after cochlear implantation.
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关键期听觉剥夺引起的中枢可塑性和功能障碍
影响因子：
3.5
作者：
Chen Z;Yuan W
通讯作者：
Yuan W
影响因子：
5.3
作者：
Kumar, SS;Bacci, A;Huguenard, JR
通讯作者：
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DOI：
10.12659/msm.890965
发表时间：
2015-03-06
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
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作者：
Iwanicka-Pronicka K;Pollak A;Skórka A;Lechowicz U;Korniszewski L;Westfal P;Skarżyński H;Płoski R
通讯作者：
Płoski R
DOI：
10.1016/s0165-3806(01)00271-1
发表时间：
2001-11-26
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
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作者：
Kullmann, PHM;Kandler, K
通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
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