An extremely rich repertoire of bursting patterns during the development of cortical cultures.
An extremely rich repertoire of bursting patterns during the development of cortical cultures.
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在皮质文化的发展过程中，极富丰富的曲目爆发模式。
DOI：
10.1186/1471-2202-7-11
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发表时间：
2006-02-07
期刊：
影响因子：
2.4
通讯作者：
Potter, SM
中科院分区：
文献类型：
--
作者：
Wagenaar, DA;Pine, J;Potter, SM
我们已经收集了一组关于分离的皮质培养的多单位数据。以前关于皮层神经元分离培养的电活动发展的研究都集中在其动力学的有限方面，并且通常基于少量的观察培养。在发育的前五周，我们跟踪了58种不同密度的培养物--3000到50000个神经元，分布在30到75mm2的区域--生长在多电极阵列(MEA)上。电镀密度对发展有着深远的影响。虽然聚集尖峰的检测率与密度成线性关系，正如电极附近的细胞数量所预期的那样，但密集培养物在发育早期就开始表现出爆裂行为，而稀疏培养物则是如此。对电刺激反应的分析表明，在密集培养中，轴突生长同样更快。两周后，在大多数文化中，网络活动被人口激增所主导。与以前的报告不同的是，在整个观察期内，情况继续发展，突发模式不断变化。爆发模式非常多样，爆发间隔在1到300时S之间，不同数量的爆发在时间上聚集，在爆发期间不同的射速分布。在发育的某些阶段，爆发被组织成具有高度保守的内部结构的紧密集群。分离培养的皮质细胞表现出比先前报道的更丰富的活动模式。除了最稀疏的文化外，所有文化都表现出全球同步的爆发，但爆发模式在发展过程中发生了变化，在不同的准备工作中也有很大不同。这强调了在任何涉及神经元培养的研究中使用多种制剂的重要性--而不仅仅是一种制剂的多种培养。这些结果是基于963个半小时的录音得出的。为了鼓励对皮质细胞在体外表现出的丰富行为范围的进一步研究，我们正在向其他研究人员提供这些数据，以及便于访问的MatLab代码。
We have collected a comprehensive set of multi-unit data on dissociated cortical cultures. Previous studies of the development of the electrical activity of dissociated cultures of cortical neurons each focused on limited aspects of its dynamics, and were often based on small numbers of observed cultures. We followed 58 cultures of different densities – 3000 to 50,000 neurons on areas of 30 to 75 mm2 – growing on multi-electrode arrays (MEAs) during the first five weeks of their development. Plating density had a profound effect on development. While the aggregate spike detection rate scaled linearly with density, as expected from the number of cells in proximity to electrodes, dense cultures started to exhibit bursting behavior earlier in development than sparser cultures. Analysis of responses to electrical stimulation suggests that axonal outgrowth likewise occurred faster in dense cultures. After two weeks, the network activity was dominated by population bursts in most cultures. In contrast to previous reports, development continued with changing burst patterns throughout the observation period. Burst patterns were extremely varied, with inter-burst intervals between 1 and 300 s, different amounts of temporal clustering of bursts, and different firing rate profiles during bursts. During certain stages of development bursts were organized into tight clusters with highly conserved internal structure. Dissociated cultures of cortical cells exhibited a much richer repertoire of activity patterns than previously reported. Except for the very sparsest cultures, all cultures exhibited globally synchronized bursts, but bursting patterns changed over the course of development, and varied considerably between preparations. This emphasizes the importance of using multiple preparations – not just multiple cultures from one preparation – in any study involving neuronal cultures. These results are based on 963 half-hour-long recordings. To encourage further investigation of the rich range of behaviors exhibited by cortical cells in vitro, we are making the data available to other researchers, together with Matlab code to facilitate access.
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影响因子：
5.3
作者：
Shahaf, G;Marom, S
通讯作者：
Marom, S
影响因子：
2.5
作者：
Van Pelt, J;Corner, MA;Ramakers, GJA
通讯作者：
Ramakers, GJA
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56.9
作者：
Leinekugel, X;Khazipov, R;Buzsáki, G
通讯作者：
Buzsáki, G
影响因子：
3.5
作者：
DeMarse, TB;Wagenaar, DA;Potter, SM
通讯作者：
Potter, SM
影响因子：
2
作者：
HABETS, AMMC;VANDONGEN, AMJ;CORNER, MA
通讯作者：
CORNER, MA