Neuroendocrine Control of Follicle-Stimulating Hormone (FSH) Secretion: II. Is Follistatin-Induced Suppression of FSH Secretion Mediated via Changes in Activin Availability and Does It Involve Changes in Gonadotropin-Releasing Hormone Secretion?1
Neuroendocrine Control of Follicle-Stimulating Hormone (FSH) Secretion: II. Is Follistatin-Induced Suppression of FSH Secretion Mediated via Changes in Activin Availability and Does It Involve Changes in Gonadotropin-Releasing Hormone Secretion?1
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卵泡刺激素 (FSH) 分泌的神经内分泌控制：II。
DOI：
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发表时间：
2002
影响因子：
3.6
通讯作者：
J. V. Van Cleeff
中科院分区：
文献类型：
--
作者：
V. Padmanabhan;D. Battaglia;Morton B. Brown;F. Karsch;James S. Lee;Wenqin Pan;D. Phillips;J. V. Van Cleeff
摘要本研究的目的是确定激活素在多大程度上参与设定FSH分泌水平，以及这种调节是否包括通过GnRH分泌变化进行的调节。我们管理卵泡抑素，激活素的高亲和力结合蛋白，5卵巢切除绵羊，我们的理由是，卵泡抑素激活素的结合应降低激活素的生物利用度和FSH分泌。垂体门静脉和外周血样本，同时收集在10分钟的时间间隔为18小时，以测量GnRH，LH，FSH，和激活素和总卵泡抑素。采集6小时后，每只母羊静脉注射150 μg/kg重组人卵泡抑素-288。一周后，对相同的母羊进行第二系列相似长度的血液采集（时间对照）。垂体门静脉血中FSH水平约为外周循环中FSH水平的8倍。FSH分泌模式在时间控制期间变化最小。相比之下，卵泡抑素对FSH分泌有深刻的抑制作用。FS-288给药后的最大FSH抑制发生在垂体门静脉5-6 h（65%抑制）和外周循环9-10 h（48%抑制）。卵泡抑素对GnRH或LH分泌模式无影响。总卵泡抑素的消失（即，游离卵泡抑素加活化素结合卵泡抑素）从循环中排出的速度比单独的游离卵泡抑素慢（P < 0.05），表明部分卵泡抑素与循环活化素复合，从而降低了活化素的生物利用度。总卵泡抑素清除较慢，且卵泡抑素对GnRH分泌缺乏影响，表明激活素生物利用度的变化决定了垂体FSH分泌水平，这是一种垂体特异性效应。
Abstract The objective of the present study was to determine to what extent activin participates in setting the level of FSH secretion and if this regulation includes mediation via changes in GnRH secretion. We administered follistatin, the high-affinity binding protein for activin, to five ovariectomized sheep; we reasoned that the resultant binding of follistatin to activin should lower activin bioavailability and FSH secretion. Hypophyseal portal and peripheral blood samples were collected simultaneously at 10-min intervals for 18 h to measure GnRH, LH, FSH, and both activin-free and total follistatin. Six hours into collection, each ewe received 150 μg/kg i.v. of recombinant human follistatin-288. A week later, the same ewes were subjected to a second series of blood collections of similar length (time control). The FSH levels in pituitary portal blood were approximately 8-fold higher than those in the peripheral circulation. The FSH secretory patterns changed minimally during the time-control period. In contrast, follistatin had profound suppressive effects on FSH secretion. Maximal FSH suppression after FS-288 administration occurred at 5–6 h in the pituitary portal (65% suppression) and 9–10 h in the peripheral (48% suppression) circulation. Follistatin had no effect on GnRH or LH secretory patterns. Disappearance of total follistatin (i.e., free follistatin plus activin-bound follistatin) from the circulation was slower (P < 0.05) than that of free follistatin alone, suggesting that some of the follistatin was complexed with circulating activin, thus reducing the bioavailability of activin. The slower clearance of total follistatin and the lack of follistatin effects on GnRH secretion suggest that changes in activin bioavailability dictate the level of pituitary FSH secretion and that this is a pituitary-specific effect.
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卵巢切除大鼠中雌激素、抑制素和卵泡抑素（激活素结合蛋白）对脉动促性腺激素分泌的调节。
影响因子：
3.6
作者：
Depaolo,LV;Shimonaka,M;Ling,N
通讯作者：
Ling,N
女孩青春期血清抑制素、激活素和卵泡抑素浓度的变化。
DOI：
10.1093/humrep/15.5.1052
发表时间：
2000
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Foster,CM;Phillips,DJ;Wyman,T;Evans,LW;Groome,NP;Padmanabhan,V
通讯作者：
Padmanabhan,V
影响因子：
20.3
作者：
Alfredo Ulloa-Aguirre;A. Midgley;I. Beitins;I. Beitins;Vasantha Padmanabhan
通讯作者：
Alfredo Ulloa-Aguirre;A. Midgley;I. Beitins;I. Beitins;Vasantha Padmanabhan
影响因子：
4.8
作者：
Huey-Jing Huang;Joyce C. Wu;P. Su;Oksana V. Zhirnov;William L. Miller
通讯作者：
Huey-Jing Huang;Joyce C. Wu;P. Su;Oksana V. Zhirnov;William L. Miller
重组人抑制素 A 在成年雄性恒河猴 (Macaca mulatta) 中抑制卵泡刺激激素的时间过程。
DOI：
10.1210/endo.139.8.6125
发表时间：
1998
期刊：
Endocrinology.
影响因子：
--
作者：
Ramaswamy,S;Pohl,CR;McNeilly,AS;Winters,SJ;Plant,TM
通讯作者：
Plant,TM