The effect of testosterone withdrawal and subsequent germ cell depletion on transferrin and sulfated glycoprotein-2 messenger ribonucleic acid levels in the adult rat testis.
The effect of testosterone withdrawal and subsequent germ cell depletion on transferrin and sulfated glycoprotein-2 messenger ribonucleic acid levels in the adult rat testis.
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睾酮戒断和随后的生殖细胞耗竭对成年大鼠睾丸中转铁蛋白和硫酸化糖蛋白2信使核糖核酸水平的影响。
DOI：
10.1095/biolreprod47.1.92
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发表时间：
1992
影响因子：
3.6
通讯作者：
Zirkin,BR
中科院分区：
文献类型：
--
作者：
Roberts,KP;Santulli,R;Seiden,J;Zirkin,BR
我们研究了睾丸内睾酮浓度降低和生殖细胞数量减少对成年大鼠睾丸中转铁蛋白mRNA和硫酸化糖蛋白（SGP）-2 mRNA水平的影响。完整的大鼠接受睾酮和雌二醇填充胶囊的植入物，以抑制垂体的LI-I分泌，从而抑制睾丸间质细胞睾酮的产生。睾丸内睾酮水平在3天内下降70%至20 ng/tnl，进一步下降至ap。在14天时，propylene 15 ng/ml，随后达到约10 ng/ml的最小值。相反，每个睾丸的细长精子细胞的数量在14天内保持不变，然后在14 - 28天下降到低于正常的20%，并在植入后56天达到零。同样，粗线期精母细胞和圆形精子细胞仅在植入后14天下降。睾丸RNA的北方杂交结果显示，移植后14 ~ 28天，每个睾丸的支持细胞转铁蛋白mRNA明显减少。然而，SGP-2 mRNA/睾丸在实验的时间过程中是不变的。转铁蛋白mRNA的减少，伴随着生殖细胞的损失，表明这种mRNA是由睾丸中的生殖细胞的数量，而不是直接由睾酮调节。相反，在睾丸内睾酮减少和随后的生殖细胞丢失的情况下，SGP-2 mRNA的恒定水平表明该mRNA在成年大鼠睾丸中组成性维持。
We have examined the effects of decreasing intratesticular testosterone concentration and of decreasing germ cell number on levels of transferrin mRNA and sulfated glycoprotein(SGP)-2 mRNA in the adult rat testis. Intact rats received implants of testosterone- and estradiol-filled capsules to suppress LI-I secretion from the pituitary, thereby suppressing Leydig cell testosterone production. The levels of intratesticular testosterone declined 70% to 20 ng/tnl within 3 days, were reduced further to ap. proximately 15 ng/ml by 14 days, and subsequently reached a minimum of about 10 ng/ml. In contrast, the number of elongated spermatids per testis remained unchanged through 14 days, then declined to fewer than 20% of normal between 14 and 28 days, and reached zero by 56 days postimplantation. Likewise, both pachytene spermatocytes and round spermatids declined only after 14 days postimplantation. Northern blots of testicular RNA showed that Sertoli cell transferrin mRNA per testis decreased markedly between 14 and 28 days postimplantation. However, SGP-2 mRNA per testis was unchanged over the time course of the experiment. The decrease in transferrin mRNA, concomitant with germ cell loss, suggests that this mRNA is regulated by the number of germ cells in the testis and not directly by testosterone. In contrast, the constant level of SGP-2 mRNA in the face of reduced intratesticular testosterone and the subsequent loss of germ cells suggests that this mRNA is constitutively maintained in the adult rat testis.
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