Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review.

Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review.
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基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)分析鉴定病原微生物:综述。

DOI:
10.3390/microorganisms9071536
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发表时间:
2021-07-19
期刊:
影响因子:
4.5
通讯作者:
Hsueh PR
Hsueh PR
中科院分区:
生物学3区
文献类型:
--
作者:
Chen XF;Hou X;Xiao M;Zhang L;Cheng JW;Zhou ML;Huang JJ;Zhang JJ;Xu YC;Hsueh PR

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自2010年以来,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)已用于临床微生物学领域。与传统的生化鉴定技术相比,MALDI-TOF MS具有方便、快速、准确、成本低等优点。随着样品制备、数据库丰富和算法优化的发展,MALDI-TOF MS鉴定的准确性和速度不断提高。MALDI-TOF MS在鉴定培养菌落和快速检测样品方面显示出有希望的结果。MALDI-TOF MS在快速检测高毒力和耐药性病原体方面具有重要的研究应用。在这里,我们提出了一个科学的审查,评估MALDI-TOF MS在识别临床病原微生物的性能。MALDI-TOF MS是鉴定临床微生物的有前途的工具,尽管某些方面仍需要改进。
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.
比较两种常规表型方法和两个MALDI-TOF MS系统与DNA测序分析的准确性,以正确识别临床上遇到的酵母菌。
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