Forskolin inducibility and tissue-specific expression of the fibronectin promoter.
Forskolin inducibility and tissue-specific expression of the fibronectin promoter.
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毛喉素诱导性和纤连蛋白启动子的组织特异性表达。
DOI：
10.1128/mcb.9.4.1498-1506.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Bourgeois,S
中科院分区：
文献类型：
--
作者：
Dean,DC;Blakeley,MS;Newby,RF;Ghazal,P;Hennighausen,L;Bourgeois,S
HT-1080和JEG-3细胞中cAMP诱导纤维连接蛋白（FN）的机制不同（D. C.迪恩河F.纽比和S. Bourgeois，J.Cell Biol.106：2159 - 2170，1988）。在纤维肉瘤细胞系HT-1080中，诱导需要蛋白质合成和12至24小时的滞后期。在绒毛膜癌细胞系JEG-3中，不需要蛋白质合成，并且诱导在24小时前达到峰值，此后下降。我们表明，FN启动子在体外转录和转录起始于适当的网站。基于用这些细胞和FN启动子构建的转染实验，在人FN基因上游的-157和-188碱基对之间鉴定了cAMP反应元件（CRE）。该序列也赋予cAMP诱导在疱疹病毒胸苷激酶启动子的细胞系中，当它被放置在一个胸苷激酶-氯霉素乙酰转移酶融合基因的上游。DNA酶I保护分析和凝胶阻滞实验表明，CRE被HT-1080和JEG-3细胞以及NIH 3T3细胞中存在的蛋白质结合。多个蛋白质-CRE复合物通过凝胶阻滞与两种细胞系的提取物解析。毛喉素治疗这些细胞没有改变定性或定量的模式，观察到的CRE结合蛋白。FN启动子在HT-1080中的活性比在JEG-3细胞中的活性高至少10倍，即使在JEG-3细胞中FN生物合成的速率和累积的FN mRNA的水平都大于HT-1080细胞中的那些。HT-1080和JEG-3细胞中启动子活性的差异由位于位置-510和-56之间的序列介导。当将该构建体转染到HT-1080细胞中时，FN启动子从位置-510至-56的缺失导致启动子活性降低约30倍，并且在NIH 3T3细胞中观察到类似的结果;然而，在JEG-3细胞中观察到不到2倍的效果。这些研究的结果表明，有一定程度的FN基因表达的组织特异性，并揭示cAMP诱导介导的，在一定程度上，由相同的元素（CRE）在HT-1080和JEG-3细胞。
The mechanism of cyclic AMP (cAMP) induction of fibronectin (FN) in HT-1080 and JEG-3 cells differs (D. C. Dean, R. F. Newby, and S. Bourgeois, J. Cell Biol. 106:2159-2170, 1988). In the fibrosarcoma cell line HT-1080, induction requires both protein synthesis and a lag period of 12 to 24 h. In the choriocarcinoma cell line JEG-3, protein synthesis is not required and induction peaks before 24 h, declining thereafter. We show that the FN promoter is transcribed in vitro and that the transcripts initiate at the proper site. Based on transfection experiments with these cells and FN promoter constructions, a cAMP-responsive element (CRE) was identified between -157 and -188 base pairs upstream of the human FN gene. This sequence also conferred cAMP inducibility in both cell lines on the herpesvirus thymidine kinase promoter when it was placed upstream of a thymidine kinase-chloramphenicol acetyltransferase fusion gene. DNase I protection analysis and gel retardation experiments revealed that the CRE was bound by a protein(s) that was present in both HT-1080 and JEG-3 cells as well as in NIH 3T3 cells. Multiple protein-CRE complexes were resolved by gel retardation with extracts of both cell lines. Forskolin treatment of these cells did not alter qualitatively or quantitatively the pattern of CRE-binding proteins that was observed. The FN promoter was at least 10 times more active in HT-1080 than in JEG-3 cells, even though in JEG-3 cells both the rate of FN biosynthesis and the level of accumulated FN mRNA were greater than those in HT-1080 cells. The difference in promoter activity in HT-1080 and JEG-3 cells was mediated by sequences that were located between positions -510 and -56. Deletion of the FN promoter from positions –510 to –56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells. Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
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转化细胞克隆中转染的 c-Ha-rasVal 12 癌基因的剂量效应。
影响因子：
3.7
作者：
Lea Sistonen;Jorma Keski;Ismo Ulmanen;E. Hölttä;Bo;Kari Alitalo
通讯作者：
Kari Alitalo
高效地将基因转移到哺乳动物细胞中。
DOI：
10.1098/rstb.1984.0137
发表时间：
1984
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Cornelia M. Gorman;David P. Lane;Peter W.J. Rigby
通讯作者：
Peter W.J. Rigby
影响因子：
56.9
作者：
EDLUND, T;WALKER, MD;RUTTER, WJ
通讯作者：
RUTTER, WJ
DOI：
10.1073/pnas.83.18.6682
发表时间：
1986-09-01
影响因子：
11.1
作者：
MONTMINY, MR;SEVARINO, KA;GOODMAN, RH
通讯作者：
GOODMAN, RH
纤连蛋白基因的组织为进化过程中的外显子改组提供了证据。
DOI：
10.1002/j.1460-2075.1987.tb02545.x
发表时间：
1987
期刊：
The EMBO journal
影响因子：
--
作者：
Patel,RS;Odermatt,E;Schwarzbauer,JE;Hynes,RO
通讯作者：
Hynes,RO