A B Functional Gene Cloned from Lily Encodes an Ortholog of Arabidopsis PISTILLATA (PI)

A B Functional Gene Cloned from Lily Encodes an Ortholog of Arabidopsis PISTILLATA (PI)
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从百合中克隆的 B 功能基因编码拟南芥 PISTILLATA (PI) 的直向同源物

DOI:
10.1007/s11105-010-0193-1
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发表时间:
2010-03
影响因子:
2.1
通讯作者:
Xi, Mengli
Xi, Mengli
中科院分区:
生物学4区
文献类型:
--
作者:
Hu, Xiaohua;Shi, Jisen;Wu, Xiaoping;Luo, Zewei;Xi, Mengli

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拟南芥和金鱼草花发育的经典ABC模型为阐明花发育和分化的生物学过程提供了理论依据,并可用于其它重要观赏植物花结构的调控。本研究从麝香百合中克隆了一个B功能基因LLGLO 1。RT-PCR分析阐明了LLGLO 1的时空表达模式。该基因在1、2和3轮器官中表达量很高,外轮花被片,内轮花被片和雄蕊。通过在模式植物拟南芥中的异位表达来检测LLGLO 1的遗传效应。转化植株的第一轮萼片同源异型转化为瓣状萼片,这与35 S::PI转基因植株相似。因此LLGLO 1可能是GLO/PI亚家族基因中的一员,在花发育中起重要作用。
The classical ABC model proposed for flower development in Arabidopsis and Antirrhinum appropriately sheds light on the biological process of flower development and differentiation and serves in manipulating the floral structure of other important ornamental plants. In this study, LLGLO1, a B functional gene from Lilium longiflorum was isolated and characterized. RT-PCR analysis elucidated that temporal and spatial expression pattern of LLGLO1. This putative gene was strongly expressed in 1, 2, and 3 whorl organs, i.e., outer whorl tepals, inner whorl tepals, and stamens. Genetic effect of LLGLO1 was assayed by ectopic expression in model plant Arabidopsis. Transformed plants showed homeotic transformation of sepals into petaloid sepals in the first whorl, which is similar to the transgenic plants of 35S::PI. So LLGLO1 was one member of GLO/PI sub-family gene to function in flower development.
DOI: 10.1016/s0168-9452(99)00206-x
发表时间: 2000-02-21
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