TRPV1 expression in acupuncture points: response to electroacupuncture stimulation.
TRPV1 expression in acupuncture points: response to electroacupuncture stimulation.
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DOI：
10.1016/j.jchemneu.2011.01.001
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发表时间：
2011-04
影响因子：
2.8
通讯作者：
Ma SX
中科院分区：
文献类型：
--
作者：
Abraham TS;Chen ML;Ma SX
本研究旨在检测瞬时受体电位香草样蛋白1 （TRPV1）受体免疫反应性在穴位（腧穴）的分布，并探讨电针（EA）刺激对TRPV1表达的影响。对麻醉大鼠进行bl40的EA刺激，每次20 min，每次间隔80 min。用针对TRPV1的抗体进行免疫标记，检测含有bl40的皮肤切片及其非经络对照。穴位组表达TRPV1的皮下神经纤维数量明显高于对照组（p<0.01）。皮下神经纤维显示TRPV1与外周素（c -纤维和a - δ纤维的标记物）共定位。穴位电刺激后，神经纤维中TRPV1的表达显著升高（p<0.01）。而非穴位经络和非经络对照皮肤的上调则缺乏统计学意义。TRPV1和神经元一氧化氮合酶（nNOS）的双重免疫染色显示它们在真皮下神经纤维和真皮结缔组织细胞中共定位。这些结果表明,高TRPV1的表达赋予nNOS皮下形成神经纤维存在于穴位的表达式是增加了EA。我们认为更高的TRPV1的表达在皮下形成神经纤维及其upregulation EA刺激可能发挥关键作用在调解EA信号的转导中枢神经系统,及其在皮下结缔组织细胞表达可能扮演一个角色在进行EA的局部作用。
The present study was to examine the distribution of transient receptor potential vanilloid type-1 (TRPV1) receptor immunoreactivity in the acupuncture points (acupoint), and determine the influences of electroacupuncture (EA) stimulation on TRPV1 expression. EA stimulation of BL 40 was conducted in two sessions of 20 min separated by an 80 min interval in anesthetized rats. Sections of skin containing BL 40, and its non-meridian control were examined by immunolabeling with antibodies directed against TRPV1. Without EA, the number of subepidermal nerve fibers expressing TRPV1 was higher in the acupoint than in non-acupoint control skin (p<0.01). The subepidermal nerve fibers showed the co-localization of TRPV1 with peripherine, a marker for the C-fibers and A- δ fibers. The expression of TRPV1 in nerve fibers is significantly increased by EA stimulation in acupoints (p<0.01). However the upregulation in the non acupoint meridian and the non-meridian control skin were short of statistical significance. Double immunostaining of TRPV1 and neuronal nitric oxide synthase (nNOS) revealed their co-localizationin both the subepidermal nerve fibers and in the dermal connective tissue cells. These results show that a high expression of TRPV1 endowed with nNOS in subepidermal nerve fibers exist in the acupoints and the expression is increased by EA. We conclude that the higher expression of TRPV1 in the subepidermal nerve fibers and its upregulation after EA stimulation may play a key role in mediating the transduction of EA signals to the CNS, and its expression in the subepidermal connective tissue cells may play a role in conducting the local effect of the EA.
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