High-throughput in vivo analysis of gene expression in Caenorhabditis elegans.
High-throughput in vivo analysis of gene expression in Caenorhabditis elegans.
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DOI：
10.1371/journal.pbio.0050237
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发表时间：
2007-09
期刊：
影响因子：
9.8
通讯作者：
Moerman DG
中科院分区：
文献类型：
--
作者：
Hunt-Newbury R;Viveiros R;Johnsen R;Mah A;Anastas D;Fang L;Halfnight E;Lee D;Lin J;Lorch A;McKay S;Okada HM;Pan J;Schulz AK;Tu D;Wong K;Zhao Z;Alexeyenko A;Burglin T;Sonnhammer E;Schnabel R;Jones SJ;Marra MA;Baillie DL;Moerman DG
利用DNA序列5 '打开阅读框，我们构建了绿色荧光蛋白（GFP）融合体，并生成了1886个特定基因的时空组织表达谱。这项工作涵盖了该生物体中已识别的所有基因的10%左右。对表达gfp的野生型动物从胚胎到成虫的各个发育阶段进行分析。我们已经确定了5 ' DNA区域在该生物的所有发育阶段和38种不同的细胞和组织类型中调节表达。在已确定的调控区域中，有在所有细胞、特定组织、组织组合和单细胞中调控表达的序列。我们在秀丽隐杆线虫中检测的大多数基因都与人类有同源关系。本项目生成的所有图像和表情模式数据均可通过WormAtlas （http://gfpweb.aecom.yu.edu/index）和WormBase （http://www.wormbase.org）获取。了解蛋白质的表达位置为了解其潜在功能提供了重要线索。尽管这一信息至关重要，但在任何后生动物中，我们只有一小部分基因的完整发育表达谱。在这里，我们生成了秀丽隐杆线虫所有基因中10%的空间和时间组织表达谱。我们分析了从胚胎到成虫的每个发育阶段表达与绿色荧光蛋白融合的基因调控元件的蠕虫。在已确定的调控区域中，有在所有细胞、特定组织、组织组合和单细胞中调控表达的序列。我们在秀丽隐杆线虫中检测的大多数基因都与人类有同源关系。我们对如此多基因的复杂表达模式的分析不仅可以促进秀丽隐杆线虫的功能分析，还可以为解码所有生物中控制基因表达的信息等级奠定基础。利用DNA序列5'打开阅读框，作者构建了绿色荧光蛋白融合体，并生成了秀丽隐杆线虫所有基因中10%的时空组织表达谱。
Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 5′ DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org). Knowing where a protein is expressed provides an important clue about its potential function. As critical as this information is, we have complete developmental expression profiles for only a small fraction of all genes expressed in any metazoan. Here, we have generated spatial and temporal tissue expression profiles for 10% of all genes in the nematode Caenorhabditis elegans. Worms expressing putative gene regulatory elements fused with green fluorescent protein were analyzed at each stage of development from embryo to adult. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. Our analysis of complex expression patterns for so many genes may not only facilitate functional analysis in C. elegans, but also create a foundation for decoding the informational hierarchies governing gene expression in all organisms. Using DNA sequences 5' to open reading frames, the authors construct green fluorescent protein fusions and generate spatial and temporal tissue expression profiles for 10% of all genes in the nematode Caenorhabditis elegans.
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影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC
影响因子：
9.2
作者：
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通讯作者：
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7
作者：
GuhaThakurta, D;Schriefer, LA;Stormo, GD
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影响因子：
64.8
作者：
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影响因子：
10.5
作者：
Fukushige, Tetsunari;Brodigan, Thomas M.;Krause, Michael
通讯作者：
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