Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S.
Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S.
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DOI：
10.1186/1471-2180-8-102
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发表时间：
2008-06-24
期刊：
影响因子：
4.2
通讯作者：
McDowell DA
中科院分区：
文献类型：
--
作者：
Giotis ES;Muthaiyan A;Blair IS;Wilkinson BJ;McDowell DA
关于单核细胞增生李斯特菌的耐碱性反应（AlTR）的信息非常有限。用蛋白质合成抑制剂氯霉素处理碱适应细胞表明，AlTR至少部分依赖于蛋白质。为了获得更全面的角度对AlTR的生理和调节，我们比较了差异的基因表达和蛋白质含量的细胞适应在pH 9.5和非适应细胞（pH 7.0）使用互补DNA（cDNA）微阵列和二维（2D）凝胶电泳，（结合质谱）分别。在本研究中，L.单核细胞增多症显示在暴露于弱碱（pH 9.5）1小时后表现出显著的AlTR，其能够保护细胞免受随后的致死性碱胁迫（pH 12.0）。适应性细胞内基因表达涉及与毒力、一般应激反应、细胞分裂和细胞壁结构变化相关的基因，并且包括许多功能未知的基因。cDNA阵列和2D凝胶电泳结果之间观察到的变异性可能是由翻译后修饰。有趣的是，一些碱诱导的基因/蛋白质可以提供交叉保护重叠到其他类型的胁迫。因此，碱性pH提供L。在某些情况下，具有非特异性多重应激抗性的单核细胞增多症可能对于在人类胃肠道以及碱性条件占主导地位的食品加工系统中的生存至关重要。本研究有力地证明了L.单核细胞增生的功能是使过量的碱化和能量消耗最小化，同时调动可用的碳源。
Information regarding the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes is very limited. Treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the AlTR is at least partially protein-dependent. In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively. In this study, L. monocytogenes was shown to exhibit a significant AlTR following a 1-h exposure to mild alkali (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Adaptive intracellular gene expression involved genes that are associated with virulence, the general stress response, cell division, and changes in cell wall structure and included many genes with unknown functions. The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. Interestingly, several alkali induced genes/proteins can provide a cross protective overlap to other types of stresses. Alkali pH provides therefore L. monocytogenes with nonspecific multiple-stress resistance that may be vital for survival in the human gastrointestinal tract as well as within food processing systems where alkali conditions prevail. This study showed strong evidence that the AlTR in L. monocytogenes functions as to minimize excess alkalisation and energy expenditures while mobilizing available carbon sources.
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影响因子：
64.8
作者：
HYDE, SC;EMSLEY, P;HIGGINS, CF
通讯作者：
HIGGINS, CF
影响因子：
2.8
作者：
Hansen, JM;Gernep-Smidt, P;Bruun, B
通讯作者：
Bruun, B
影响因子：
3.6
作者：
Cheng, JB;Guffanti, AA;Krulwich, TA
通讯作者：
Krulwich, TA
影响因子：
4.4
作者：
Flahaut, S;Hartke, A;Auffray, Y
通讯作者：
Auffray, Y
影响因子：
3.5
作者：
KAKINUMA, Y;IGARASHI, K
通讯作者：
IGARASHI, K