Design Considerations for Silica-Particle-Doped Nitric-Oxide-Releasing Polyurethane Glucose Biosensor Membranes.
Design Considerations for Silica-Particle-Doped Nitric-Oxide-Releasing Polyurethane Glucose Biosensor Membranes.
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DOI：
10.1021/acssensors.6b00623
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发表时间：
2017-01-27
期刊：
影响因子：
8.9
通讯作者：
Schoenfisch MH
中科院分区：
文献类型：
--
作者：
Soto RJ;Schofield JB;Walter SE;Malone-Povolny MJ;Schoenfisch MH
释放一氧化氮（NO）的聚合物已被证明可用于改善体内葡萄糖生物传感器的生物相容性。不幸的是，NO供体从聚合物基质中的浸出仍然是NO释放膜的关键设计缺陷。在此，一个工具箱的NO-释放二氧化硅纳米粒子（SNP）被用来系统地评估SNP浸出的生物医学级聚氨酯传感器膜的各种选择。葡萄糖传感器的分析性能和NO-释放动力学从传感器膜也进行了评价，作为一个功能的颗粒和聚氨酯化学。由于NO供体改性的两性离子性质，用N-二醇二氮烯鎓 NO供体改性的颗粒易于从PU膜中浸出。在低吸水PU中，浸出最小化（在1个月内截留的二氧化硅<5%）。然而，用中性S-亚硝基硫醇（RSNO）NO供体修饰SNP导致双相浸出行为。具有低烷醇含量（<3.0重量%硫）的颗粒从水凝胶PU制剂（HP-93 A-100 PU）中过度浸出，而具有较大硫醇改性程度的颗粒不从任何测试的PU中浸出。使用掺杂RSNO修饰SNP的优化HP-93 A-100 PU膜作为外部葡萄糖扩散限制层开发了一种功能性葡萄糖传感器。实现的传感器设计在PBS中孵育2周后对葡萄糖的生理浓度（最小1-21 mM）线性响应，并在>0.8 pmol cm-2 s-1下释放NO长达6 d，没有检测到（<0.6%）颗粒浸出。
Nitric oxide (NO)-releasing polymers have proven useful for improving the biocompatibility of in vivo glucose biosensors. Unfortunately, leaching of the NO donor from the polymer matrix remains a critical design flaw of NO-releasing membranes. Herein, a toolbox of NO-releasing silica nanoparticles (SNPs) was utilized to systematically evaluate SNP leaching from a diverse selection of biomedical-grade polyurethane sensor membranes. Glucose sensor analytical performance and NO-release kinetics from the sensor membranes were also evaluated as a function of particle and polyurethane chemistries. Particles modified with N-diazeniumdiolate NO donors were prone to leaching from PU membranes due to the zwitterionic nature of the NO donor modification. Leaching was minimized (<5% of the entrapped silica over 1 mo) in low water uptake PUs. However, SNP modification with neutral S-nitrosothiol (RSNO) NO donors lead to biphasic leaching behavior. Particles with low alkanethiol content (<3.0 wt% sulfur) leached excessively from a hydrogel PU formulation (HP-93A-100 PU), while particles with greater degrees of thiol modification did not leach from any of the PUs tested. A functional glucose sensor was developed using an optimized HP-93A-100 PU membrane doped with RSNO-modified SNPs as the outer, glucose diffusion-limiting layer. The realized sensor design responded linearly to physiological concentrations of glucose (min. 1–21 mM) over 2 wk incubation in PBS and released NO at >0.8 pmol cm−2 s−1 for up to 6 d with no detectable (<0.6%) particle leaching.
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影响因子：
2.9
作者：
de Oliveira, MG;Shishido, SM;Morgon, NH
通讯作者：
Morgon, NH
影响因子：
6.2
作者：
Jun, HW;Taite, LJ;West, JL
通讯作者：
West, JL
影响因子：
12.6
作者：
Koh, Ahyeon;Riccio, Daniel A.;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
7.4
作者：
Chen, XH;Matsumoto, N;Wilson, GS
通讯作者：
Wilson, GS
一氧化氮释放：第二部分。治疗应用。
影响因子：
46.2
作者：
Carpenter AW;Schoenfisch MH
通讯作者：
Schoenfisch MH