Streptococcal peptides that signal Enterococcus faecalis cells carrying the pheromone-responsive conjugative plasmid pAM373.
Streptococcal peptides that signal Enterococcus faecalis cells carrying the pheromone-responsive conjugative plasmid pAM373.
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链球菌肽向携带信息素响应接合质粒 pAM373 的粪肠球菌细胞发出信号。
DOI：
10.1111/omi.12271
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发表时间：
2019
影响因子：
3.7
通讯作者：
Mansfield,JillianM
中科院分区：
文献类型：
--
作者：
Vickerman,MMargaret;Mansfield,JillianM
信息素介导的肠球菌质粒的结合转移有助于在混合微生物群落的共同居民中传播涉及抗生素耐药性、适应性和毒力的基因。我们之前已经证明，戈登链球菌链球菌七肽s.g.cAM373 （SVFILAA）的属间信号传导诱导了聚集物质介导的交配反应，并促进质粒从携带信息素响应质粒pAM373的粪肠球菌细胞转移到产生信息素和不产生信息素的口腔链球菌受体。为了进一步研究链球菌信息素样肽，我们在链球菌CamG脂蛋白信号序列中发现了s.g.cAM373样序列及其诱导交配反应的能力。检测粪便/pAM373细胞。合成序列一致的七聚体（A/S）‐（I/V）‐F‐I‐L‐（A/V/T）‐（S/A）诱导AS‐介导的聚块。保守的信息素ABC转运体由ys编码。鉴定并确认了SGO_RS02660和SGO_RS02665位点是s.g.cAM373活性所必需的。代表性口腔血分离株培养上清液的功能分析。gordonii发现，除了编码s.g.cAM373的菌株外，编码新发现的信息素s.g.cAM373‐V （SVFILVA）的菌株SK120能够诱导肠球菌结块，而编码s.g.cAM373‐T （SVFILTA）的菌株SK6、SK8、SK9和SK86则没有引起可检测到的交配反应。编码CamG前体的异源寄主上清缺乏信息素活性，表明s.g.cAM373‐T未被有效加工和/或运输。综上所述，这些研究表明了活性信息素肽在s菌株之间的分布。并支持肠球菌-链球菌交流在促进口腔宏基因组遗传可塑性中的潜在作用。
Pheromone‐mediated conjugative transfer of enterococcal plasmids can contribute to the dissemination of genes involved in antibiotic resistance, fitness, and virulence among co‐residents of mixed microbial communities. We have previously shown that intergeneric signaling by theStreptococcus gordoniistrain Challis heptapeptide s.g.cAM373 (SVFILAA) induces an aggregation substance‐mediated mating response and facilitates plasmid transfer fromEnterococcus faecaliscells carrying the pheromone‐responsive plasmid pAM373 to both pheromone‐producing and non‐pheromone‐producing oral streptococcal recipients. To further investigate the streptococcal pheromone‐like peptides, s.g.cAM373‐like sequences were identified in the signal sequences of streptococcal CamG lipoproteins and their abilities to induce a mating response inE. faecalis/pAM373 cells were examined. Synthetic heptamers with the consensus sequence (A/S)‐(I/V)‐F‐I‐L‐(A/V/T)‐(S/A) induced AS‐mediated clumping. The conserved pheromone ABC transporter encoded byS. gordoniigenome loci SGO_RS02660 and SGO_RS02665 was identified and confirmed to be required for s.g.cAM373 activity. Functional assays of culture supernatants from representative oral and blood isolates ofS. gordoniishowed that in addition to strains encoding s.g.cAM373, strain SK120, encoding the newly identified pheromone s.g.cAM373‐V (SVFILVA), was able to induce enterococcal clumping, whereas strains SK6, SK8, SK9, and SK86 which encoded s.g.cAM373‐T (SVFILTA) did not elicit a detectable mating response. Absence of pheromone activity in supernatants of heterologous hosts encoding its CamG precursor suggested that s.g.cAM373‐T was not effectively processed and/or transported. Overall, these studies demonstrated the distribution of active pheromone peptides among strains ofS. gordonii, and support a potential role for enterococcal–streptococcal communication in contributing to genetic plasticity in the oral metagenome.
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影响因子：
4.9
作者：
Jensen, Anders;Valdorsson, Oskar;Kilian, Mogens
通讯作者：
Kilian, Mogens
影响因子：
3
作者：
Jillian M. Mansfield;Paul Herrmann;A. Jesionowski;M. Vickerman
通讯作者：
Jillian M. Mansfield;Paul Herrmann;A. Jesionowski;M. Vickerman
与粪肠球菌其他性信息素质粒编码聚集物质无关的新型粘附素Asa373的克隆和功能分析
影响因子：
3.6
作者：
A. Muscholl
通讯作者：
A. Muscholl
影响因子：
--
作者：
Sedgley, CM;Lennan, SL;Clewell, DB
通讯作者：
Clewell, DB
影响因子：
3.7
作者：
Song X;Sun J;Mikalsen T;Roberts AP;Sundsfjord A
通讯作者：
Sundsfjord A