Adaptation to Endophytic Lifestyle Through Genome Reduction by Kitasatospora sp. SUK42.
Adaptation to Endophytic Lifestyle Through Genome Reduction by Kitasatospora sp. SUK42.
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DOI：
10.3389/fbioe.2021.740722
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发表时间：
2021
影响因子：
5.7
通讯作者：
Herron PR
中科院分区：
文献类型：
--
作者：
Zin NM;Ismail A;Mark DR;Westrop G;Schniete JK;Herron PR
内生放线菌作为新型生物活性化合物的来源具有很大的潜力。为了研究内生菌产生次生代谢物的可能性，我们从Antidesma neurocarpum Miq树中回收了一种丝状微生物。经过表型分析和全基因组测序，我们证明该生物，SUK42是放线菌属Kitasatospora的成员。与其他类型菌株相比，该菌株具有较小的基因组，并且失去了与应激反应，氮代谢和次级代谢相关的代谢途径。尽管如此，SUK42可以在实验室环境中生长良好，并编码与该属其他成员一致的核心基因组。最后，与Kitasatospora的其他成员相比，SUK42编码糖次生代谢物生物合成基因簇，其中一个与醋维抑素簇相似，其产物具有α-淀粉酶抑制活性。由于寄主植物的提取物显示出这种抑制活性，这表明A. neurocarpum Miq可能由内生伴侣提供潜在的药用价值，并说明了内生菌在临床或工业应用上的开发潜力。
Endophytic actinobacteria offer great potential as a source of novel bioactive compounds. In order to investigate the potential for the production of secondary metabolites by endophytes, we recovered a filamentous microorgansism from the tree Antidesma neurocarpum Miq. After phenotypic analysis and whole genome sequencing we demonstrated that this organism, SUK42 was a member of the actinobacterial genus Kitasatospora. This strain has a small genome in comparison with other type strains of this genus and has lost metabolic pathways associated with Stress Response, Nitrogen Metabolism and Secondary Metabolism. Despite this SUK42 can grow well in a laboratory environment and encodes a core genome that is consistent with other members of the genus. Finally, in contrast to other members of Kitasatospora, SUK42 encodes saccharide secondary metabolite biosynthetic gene clusters, one of which with similarity to the acarviostatin cluster, the product of which displays α-amylase inhibitory activity. As extracts of the host plant demonstrate this inhibitory activity, it suggests that the potential medicinal properties of A. neurocarpum Miq might be provided by the endophytic partner and illustrate the potential for exploitation of endophytes for clinical or industrial uses.
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DOI：
10.1093/bioinformatics/btx610
发表时间：
2018-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Hadfield J;Croucher NJ;Goater RJ;Abudahab K;Aanensen DM;Harris SR
通讯作者：
Harris SR
影响因子：
--
作者：
Corretto E;Antonielli L;Sessitsch A;Compant S;Höfer C;Puschenreiter M;Brader G
通讯作者：
Brader G
影响因子：
14.9
作者：
Alanjary, Mohammad;Steinke, Katharina;Ziemert, Nadine
通讯作者：
Ziemert, Nadine
影响因子：
3.2
作者：
Grantcharova, N;Lustig, U;Flärdh, K
通讯作者：
Flärdh, K
DOI：
10.1007/s10482-011-9656-0
发表时间：
2012-01-01
影响因子：
2.6
作者：
Labeda, D. P.;Goodfellow, M.;Hatano, K.
通讯作者：
Hatano, K.