The balance of metagenomic elements shapes the skin microbiome in acne and health.
The balance of metagenomic elements shapes the skin microbiome in acne and health.
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DOI：
10.1038/srep39491
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发表时间：
2016-12-21
影响因子：
4.6
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Barnard E;Shi B;Kang D;Craft N;Li H
研究强调了与疾病相关的微生物在受干扰的群落中的重要性，然而，共生体的保护作用在很大程度上没有得到认识和了解。使用痤疮作为模型疾病，我们研究了当疾病和健康相关生物体在群落中共存时皮肤微生物群总体毒力特性的决定因素。通过超深宏基因组鸟枪测序，我们发现健康皮肤中丙酸杆菌和痤疮丙酸杆菌噬菌体的相对丰度较高。在痤疮患者中，物种水平和痤疮丙酸杆菌菌株水平的微生物组组成比健康个体更加多样化，毒力相关因子丰富，代谢合成基因丰度减少。根据宏基因组元素的丰度分布，我们构建了一个定量预测模型，该模型在我们的研究队列（85％）和独立样本集（86％）中对宿主皮肤的临床状态进行了高精度分类。我们的结果表明，宏基因组元素之间的平衡，而不仅仅是疾病相关菌株的存在，决定了皮肤微生物群的整体毒力特性。这项研究为痤疮发病机制的微生物机制提供了新的见解，并建议益生菌和噬菌体疗法作为潜在的痤疮治疗方法，以调节皮肤微生物群并保持皮肤健康。
Studies have emphasized the importance of disease-associated microorganisms in perturbed communities, however, the protective roles of commensals are largely under recognized and poorly understood. Using acne as a model disease, we investigated the determinants of the overall virulence property of the skin microbiota when disease- and health-associated organisms coexist in the community. By ultra-deep metagenomic shotgun sequencing, we revealed higher relative abundances of propionibacteria and Propionibacterium acnes phage in healthy skin. In acne patients, the microbiome composition at the species level and at P. acnes strain level was more diverse than in healthy individuals, with enriched virulence-associated factors and reduced abundance of metabolic synthesis genes. Based on the abundance profiles of the metagenomic elements, we constructed a quantitative prediction model, which classified the clinical states of the host skin with high accuracy in both our study cohort (85%) and an independent sample set (86%). Our results suggest that the balance between metagenomic elements, not the mere presence of disease-associated strains, shapes the overall virulence property of the skin microbiota. This study provides new insights into the microbial mechanism of acne pathogenesis and suggests probiotic and phage therapies as potential acne treatments to modulate the skin microbiota and to maintain skin health.
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影响因子：
4.5
作者：
Attaiech L;Olivier A;Mortier-Barrière I;Soulet AL;Granadel C;Martin B;Polard P;Claverys JP
通讯作者：
Claverys JP
影响因子：
--
作者：
Kasimatis, Gabriela;Fitz-Gibbon, Sorel;Li, Huiying
通讯作者：
Li, Huiying
与痤疮相关的人皮肤微生物组中的痤疮丙酸杆菌菌株群体。
影响因子：
6.5
作者：
Fitz-Gibbon, Sorel;Tomida, Shuta;Chiu, Bor-Han;Lin Nguyen;Du, Christine;Liu, Minghsun;Elashoff, David;Erfe, Marie C.;Loncaric, Anya;Kim, Jenny;Modlin, Robert L.;Miller, Jeff F.;Sodergren, Erica;Craft, Noah;Weinstock, George M.;Li, Huiying
通讯作者：
Li, Huiying
影响因子：
56.9
作者：
Golub, TR;Slonim, DK;Lander, ES
通讯作者：
Lander, ES
影响因子：
10.3
作者：
Jahns, A. C.;Lundskog, B.;Alexeyev, O. A.
通讯作者：
Alexeyev, O. A.