Lingual innervation in male and female marmosets.
Lingual innervation in male and female marmosets.
复制标题
DOI：
10.1016/j.ynpai.2023.100134
复制
发表时间：
2023-08
期刊：
影响因子：
--
通讯作者：
Ruparel, Shivani
中科院分区：
文献类型：
--
作者：
Tram, Meilinn;Ibrahim, Tarek;Hovhannisyan, Anahit;Akopian, Armen;Ruparel, Shivani
本研究描述了绒猴舌的感觉神经支配，并评估了特定轴突亚群的性别差异。数据发现，虽然性别之间存在一些相似之处，但女性表达TRPV1和小清蛋白的神经纤维比例较高。在我们对口面疼痛的理解中存在一些知识空白。其中一些包括特定组织中的外周感觉神经支配类型，性别之间的神经支配差异以及啮齿动物研究在高等动物中的验证。目前的研究通过验证非人类灵长类动物舌头组织感觉神经支配的小鼠研究以及评估性别特异性差异来解决这些差距。从未经处理的雄性和雌性绒猴中收集舌和三叉神经节，并通过免疫组织化学使用特异性标记物测试神经纤维，并量化纤维数量。我们还测试了特定亚组的神经纤维是否属于髓鞘或非髓鞘轴突。我们观察到与小鼠中的发现相似，绒猴舌受表达伤害性感受器标记物如CGRP和TRPV 1以及非伤害性感受器标记物如TrkB、小清蛋白（PV）和酪氨酸羟化酶（TH）的神经丝支配。此外，我们发现，虽然部分TrkB和PV可能是感觉纤维，TH阳性纤维主要是交感神经纤维。此外，CGRP，TrkB和TH阳性神经纤维的数量在两性之间相似。然而，我们观察到女性中有髓鞘TRPV1阳性纤维的比例高于男性，女性中PV +纤维的数量也增加。总之，这项研究首次描述了非人类灵长类动物的感觉神经支配，并评估了舌组织神经支配的性别差异，从而为未来使用新世界较小的NHP（如普通绒猴）进行口面疼痛研究奠定了基础。
The study characterizes sensory innervation in tongue of marmosets as well as evaluates sex-differences in specific axonal subgroups. Data found that while several similarities exist between sexes, females had higher proportion of nerve fibers expressing TRPV1 as well as parvalbumin. Several gaps in knowledge exists in our understanding of orofacial pain. Some of these include type of peripheral sensory innervation in specific tissues, differences in innervation between sexes and validation of rodent studies in higher order species. The current study addresses these gaps by validating mouse studies for sensory innervation of tongue tissue in non-human primates as well as assesses sex-specific differences. Tongue and trigeminal ganglia were collected from naïve male and female marmosets and tested for nerve fibers using specific markers by immunohistochemistry and number of fibers quantified. We also tested whether specific subgroups of nerve fibers belonged to myelinating or non-myelinating axons. We observed that similar to findings in mice, marmoset tongue was innervated with nerve filaments expressing nociceptor markers like CGRP and TRPV1 as well as non-nociceptor markers like TrkB, parvalbumin (PV) and tyrosine hydroxylase (TH). Furthermore, we found that while portion of TrkB and PV may be sensory fibers, TH-positive fibers were primarily sympathetic nerve fibers. Moreover, number of CGRP, TrkB and TH-positive nerve fibers were similar in both sexes. However, we observed a higher proportion of myelinated TRPV1 positive fibers in females than in males as well as increased number of PV + fibers in females. Taken together, the study for the first time characterizes sensory innervation in non-human primates as well as evaluates sex-differences in innervation of tongue tissue, thereby laying the foundation for future orofacial pain research with new world smaller NHPs like the common marmoset.
登录
查看更多内容
影响因子：
0.7
作者：
Arnold, C.;Lamp, J.;Einspanier, A.
通讯作者：
Einspanier, A.
DOI：
10.1016/j.tripleo.2005.10.041
发表时间：
2006-07-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Cuffari, Linneu;Tesseroli de Siqueira, Jose Tadeu;Rapaport, Abrao
通讯作者：
Rapaport, Abrao
影响因子：
3.6
作者：
GRINSPAN, D;BLANCO, GF;STENGEL, FM
通讯作者：
STENGEL, FM
影响因子：
16.6
作者：
Kasemeier-Kulesa, Jennifer C.;Morrison, Jason A.;Kulesa, Paul M.
通讯作者：
Kulesa, Paul M.
影响因子：
4.8
作者：
EPSTEIN, JB;STEWART, KH
通讯作者：
STEWART, KH