Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature.
Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature.
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DOI：
10.1016/j.bone.2021.116139
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发表时间：
2021-12
期刊：
影响因子：
4.1
通讯作者：
Nociti FH Jr
中科院分区：
文献类型：
--
作者：
Lira Dos Santos EJ;de Almeida AB;Chavez MB;Salmon CR;Mofatto LS;Camara-Souza MB;Tan MH;Kolli TN;Mohamed FF;Chu EY;Novaes PD;Santos ECA;Kantovitz KR;Foster BL;Nociti FH Jr
牙骨质是覆盖牙根的矿化组织，在牙周附着复合体中起作用。牙骨质细胞是细胞性牙骨质的驻留细胞，与骨细胞具有许多共同的特征，是基于载荷变化指导骨重建的机械响应细胞。我们假设，牙骨质细胞在正畸牙齿移动（OTM）过程中起着关键作用。为了验证这一假设，我们使用8周龄的雄性Wistar大鼠的OTM模型为2，7，或14天（0.5N），而卸载对侧牙齿作为对照。组织和细胞的反应进行了分析，高分辨率显微计算机断层扫描，组织学，抗酒石酸酸性磷酸酶染色的破牙细胞/破骨细胞，和透射电子显微镜。此外，激光捕获显微切割用于收集细胞牙骨质，并通过液相色谱-串联质谱法鉴定提取的蛋白质。OTM模型成功地将第一磨牙近中移动超过250 μm，14天，在近中和远中方向的牙骨质细胞和牙根吸收区域中引入少量凋亡。牙骨质细胞显示核大小和常染色质比例增加，表明细胞活性。蛋白质组学分析确定了168蛋白质的细胞牙骨质与21蛋白质只发现在OTM网站和54蛋白质只存在于对照样品。OTM下调了几种细胞外基质蛋白，包括核心蛋白聚糖、双糖链蛋白聚糖、asporin和periostin，通过免疫染色定位于牙骨质和PDL。此外，IV型胶原（COL 14 A1）是OTM下调最多（-45倍）的蛋白质，并免疫定位于牙骨质-牙本质交界处的细胞。十一角蛋白显着增加OTM，和泛角蛋白抗体表示角蛋白定位主要在上皮残留的Hertwig的上皮根鞘。这些实验为研究牙骨质细胞和细胞牙骨质对OTM的生物学反应提供了新的视角。
Cementum is a mineralized tissue that covers tooth roots and functions in the periodontal attachment complex. Cementocytes, resident cells of cellular cementum, share many characteristics with osteocytes, are mechanoresponsive cells that direct bone remodeling based on changes in loading. We hypothesized that cementocytes play a key role during orthodontic tooth movement (OTM). To test this hypothesis, we used 8-week-old male Wistar rats in a model of OTM for 2, 7, or 14 days (0.5N), whereas unloaded contralateral teeth served as controls. Tissue and cell responses were analyzed by high-resolution micro-computed tomography, histology, tartrate-resistant acid phosphatase staining for odontoclasts/osteoclasts, and transmission electron microscopy. In addition, laser capture microdissection was used to collect cellular cementum, and extracted proteins were identified by liquid chromatography coupled to tandem mass spectrometry. The OTM model successfully moved first molars mesially more than 250 μm by 14 days introducing apoptosis in a small number of cementocytes and areas of root resorption on mesial and distal aspects. Cementocytes showed increased nuclear size and proportion of euchromatin suggesting cellular activity. Proteomic analysis identified 168 proteins in cellular cementum with 21 proteins found only in OTM sites and 54 proteins only present in control samples. OTM-down-regulated several extracellular matrix proteins, including decorin, biglycan, asporin, and periostin, localized to cementum and PDL by immunostaining. Furthermore, type IV collagen (COL14A1) was the protein most down-regulated (−45-fold) by OTM and immunolocalized to cells at the cementum-dentin junction. Eleven keratins were significantly increased by OTM, and a pan-keratin antibody indicated keratin localization primarily in epithelial remnants of Hertwig’s epithelial root sheath. These experiments provide new insights into biological responses of cementocytes and cellular cementum to OTM.
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