Pathogenic influenza B virus in the ferret model establishes lower respiratory tract infection.
Pathogenic influenza B virus in the ferret model establishes lower respiratory tract infection.
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DOI：
10.1099/vir.0.064352-0
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Kelvin DJ
中科院分区：
文献类型：
--
作者：
Huang SSH;Banner D;Paquette SG;Leon AJ;Kelvin AA;Kelvin DJ
B型流感病毒在流感季节变得越来越突出。B型流感感染通常被认为是一种轻微的疾病，受到的关注比A型流感少，但在世界上的几个地区，B型流感感染引起的流感发病率占总流感发病率的20%至50% %。虽然有越来越多的证据表明乙型流感患者患有中下呼吸道疾病，如支气管炎和肺炎，但对最近的乙型流感病毒的发病机制知之甚少。在雪貂模型中，我们研究了代表当前两个共同循环的B血统(B/Yamagata和B/Victoria)的菌株感染的临床和病理特征。具体地说，我们研究了两个B/Victoria菌株(B/Brisbane/60/2008和B/玻利维亚/1526/2010)和两个B/Yamagata菌株(B/佛罗里达/04/2006和B/威斯康星/01/2010)在雪貂中的感染情况，观察了菌株特异性而不是谱系特异性的致病性。我们发现B/Brisbane/60/2008引起了最严重的临床疾病，B/Brisbane/60/2008和B/Yamagata菌株引起了中下呼吸道的病变。重要的是，B/Brisbane/60/2008确立了具有高病毒负担的有效下呼吸道感染。我们的系统发育分析表明，最近的B型流感病毒之间发生了深刻的重新分类，这表明B/Brisbane/60/2008的基因构成不同于其他毒株。这可能解释了雪貂感染后致病性差异的原因。
Influenza B viruses have become increasingly more prominent during influenza seasons. Influenza B infection is typically considered a mild disease and receives less attention than influenza A, but has been causing 20 to 50 % of the total influenza incidence in several regions around the world. Although there is increasing evidence of mid to lower respiratory tract diseases such as bronchitis and pneumonia in influenza B patients, little is known about the pathogenesis of recent influenza B viruses. Here we investigated the clinical and pathological profiles of infection with strains representing the two current co-circulating B lineages (B/Yamagata and B/Victoria) in the ferret model. Specifically, we studied two B/Victoria (B/Brisbane/60/2008 and B/Bolivia/1526/2010) and two B/Yamagata (B/Florida/04/2006 and B/Wisconsin/01/2010) strain infections in ferrets and observed strain-specific but not lineage-specific pathogenicity. We found B/Brisbane/60/2008 caused the most severe clinical illness and B/Brisbane/60/2008 and the B/Yamagata strains instigated pathology in the middle to lower respiratory tract. Importantly, B/Brisbane/60/2008 established efficient lower respiratory tract infection with high viral burden. Our phylogenetic analyses demonstrate profound reassortment among recent influenza B viruses, which indicates the genetic make-up of B/Brisbane/60/2008 differs from the other strains. This may explain the pathogenicity difference post-infection in ferrets.
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影响因子：
6.7
作者：
Hamelin ME;Baz M;Abed Y;Couture C;Joubert P;Beaulieu E;Bellerose N;Plante M;Mallett C;Schumer G;Kobinger GP;Boivin G
通讯作者：
Boivin G
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48
作者：
Langmead, Ben;Salzberg, Steven L.
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Salzberg, Steven L.
影响因子：
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作者：
Huang SS;Banner D;Fang Y;Ng DC;Kanagasabai T;Kelvin DJ;Kelvin AA
通讯作者：
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影响因子：
28.2
作者：
Gutierrez-Pizarraya, A.;Perez-Romero, P.;Cordero, E.
通讯作者：
Cordero, E.
影响因子：
4.6
作者：
Huang, Stephen S. H.;Lin, Zhen;Kelvin, Alyson A.
通讯作者：
Kelvin, Alyson A.