Contractile and mechanical properties of epithelia with perturbed actomyosin dynamics.
Contractile and mechanical properties of epithelia with perturbed actomyosin dynamics.
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DOI：
10.1371/journal.pone.0095695
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gorfinkiel N
中科院分区：
文献类型：
--
作者：
Fischer SC;Blanchard GB;Duque J;Adams RJ;Arias AM;Guest SD;Gorfinkiel N
Mechanics has an important role during morphogenesis, both in the generation of forces driving cell shape changes and in determining the effective material properties of cells and tissues. Drosophila dorsal closure has emerged as a reference model system for investigating the interplay between tissue mechanics and cellular activity. During dorsal closure, the amnioserosa generates one of the major forces that drive closure through the apical contraction of its constituent cells. We combined quantitation of live data, genetic and mechanical perturbation and cell biology, to investigate how mechanical properties and contraction rate emerge from cytoskeletal activity. We found that a decrease in Myosin phosphorylation induces a fluidization of amnioserosa cells which become more compliant. Conversely, an increase in Myosin phosphorylation and an increase in actin linear polymerization induce a solidification of cells. Contrary to expectation, these two perturbations have an opposite effect on the strain rate of cells during DC. While an increase in actin polymerization increases the contraction rate of amnioserosa cells, an increase in Myosin phosphorylation gives rise to cells that contract very slowly. The quantification of how the perturbation induced by laser ablation decays throughout the tissue revealed that the tissue in these two mutant backgrounds reacts very differently. We suggest that the differences in the strain rate of cells in situations where Myosin activity or actin polymerization is increased arise from changes in how the contractile forces are transmitted and coordinated across the tissue through ECadherin-mediated adhesion. Altogether, our results show that there is an optimal level of Myosin activity to generate efficient contraction and suggest that the architecture of the actin cytoskeleton and the dynamics of adhesion complexes are important parameters for the emergence of coordinated activity throughout the tissue.
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影响因子：
2
作者：
Ma, Xiaoyan;Lynch, Holley E.;Hutson, M. Shane
通讯作者：
Hutson, M. Shane
影响因子：
2.7
作者：
Kim, YS;Fritz, JL;VanBerkum, MFA
通讯作者：
VanBerkum, MFA
影响因子：
4.6
作者：
Homem, Catarina C. F.;Peifer, Mark
通讯作者：
Peifer, Mark
影响因子：
7.2
作者：
Cavey, Matthieu;Lecuit, Thomas
通讯作者：
Lecuit, Thomas
DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA