Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells.
Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells.
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DOI：
10.1038/s41598-018-26156-3
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发表时间：
2018-05-23
影响因子：
4.6
通讯作者：
Miriuka SG
中科院分区：
文献类型：
--
作者：
Garate X;La Greca A;Neiman G;Blüguermann C;Santín Velazque NL;Moro LN;Luzzani C;Scassa ME;Sevlever GE;Romorini L;Miriuka SG
MicroRNA是一类小的非编码RNA，参与许多细胞功能相关基因表达的转录后调控。我们对多能干细胞的心脏分化进行了小RNA测序分析。我们的分析确定了一些新的方面microRNA表达在这个分化过程中。首先，我们描述了microRNA的动态表达谱，其中一些microRNA根据其表达水平进行聚类。其次，我们描述了广泛的网络isomiR和阿达尔修饰。第三，我们鉴定了参与心脏谱系建立的microRNA家族和簇，并基于这些组定义了mirRNAome。最后，我们能够通过将表达的microRNA与其他成熟细胞进行比较来确定与心肌细胞相关的更准确的miRNAome。MicroRNA以复杂和相互关联的方式发挥作用，因此有必要进行全局分析以更好地了解它们的作用。我们的数据扩展了microRNA的知识及其在心肌发生中的意义。
MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We performed a small-RNAseq analysis of cardiac differentiation from pluripotent stem cells. Our analyses identified some new aspects about microRNA expression in this differentiation process. First, we described a dynamic expression profile of microRNAs where some of them are clustered according to their expression level. Second, we described the extensive network of isomiRs and ADAR modifications. Third, we identified the microRNAs families and clusters involved in the establishment of cardiac lineage and define the mirRNAome based on these groups. Finally, we were able to determine a more accurate miRNAome associated with cardiomyocytes by comparing the expressed microRNAs with other mature cells. MicroRNAs exert their effect in a complex and interconnected way, making necessary a global analysis to better understand their role. Our data expands the knowledge of microRNAs and their implications in cardiomyogenesis.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
12.4
作者：
通讯作者：
--
影响因子：
10.5
作者：
Chong, Mark M. W.;Zhang, Guoan;Littman, Dan R.
通讯作者：
Littman, Dan R.
影响因子：
56.9
作者：
Giraldez, AJ;Mishima, Y;Schier, AF
通讯作者：
Schier, AF
DOI：
10.1142/s0219720005001375
发表时间：
2005-08-01
影响因子：
1
作者：
Futschik, Matthias E.;Carlisle, Bronwyn
通讯作者：
Carlisle, Bronwyn