Epstein-Barr virus inactivates the transcriptome and disrupts the chromatin architecture of its host cell in the first phase of lytic reactivation.
Epstein-Barr virus inactivates the transcriptome and disrupts the chromatin architecture of its host cell in the first phase of lytic reactivation.
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DOI：
10.1093/nar/gkab099
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发表时间：
2021-04-06
影响因子：
14.9
通讯作者：
Hammerschmidt W
中科院分区：
文献类型：
--
作者：
Buschle A;Mrozek-Gorska P;Cernilogar FM;Ettinger A;Pich D;Krebs S;Mocanu B;Blum H;Schotta G;Straub T;Hammerschmidt W
Epstein-Barr virus (EBV), a herpes virus also termed HHV 4 and the first identified human tumor virus, establishes a stable, long-term latent infection in human B cells, its preferred host. Upon induction of EBV’s lytic phase, the latently infected cells turn into a virus factory, a process that is governed by EBV. In the lytic, productive phase, all herpes viruses ensure the efficient induction of all lytic viral genes to produce progeny, but certain of these genes also repress the ensuing antiviral responses of the virally infected host cells, regulate their apoptotic death or control the cellular transcriptome. We now find that EBV causes previously unknown massive and global alterations in the chromatin of its host cell upon induction of the viral lytic phase and prior to the onset of viral DNA replication. The viral initiator protein of the lytic cycle, BZLF1, binds to >105 binding sites with different sequence motifs in cellular chromatin in a concentration dependent manner implementing a binary molar switch probably to prevent noise-induced erroneous induction of EBV’s lytic phase. Concomitant with DNA binding of BZLF1, silent chromatin opens locally as shown by ATAC-seq experiments, while previously wide-open cellular chromatin becomes inaccessible on a global scale within hours. While viral transcripts increase drastically, the induction of the lytic phase results in a massive reduction of cellular transcripts and a loss of chromatin-chromatin interactions of cellular promoters with their distal regulatory elements as shown in Capture-C experiments. Our data document that EBV’s lytic cycle induces discrete early processes that disrupt the architecture of host cellular chromatin and repress the cellular epigenome and transcriptome likely supporting the efficient de novo synthesis of this herpes virus.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
与染色质环相关的远距离增强子可以调节分化角蛋白形成细胞中肽基金氨酸脱氨酶3基因。
影响因子：
3.7
作者：
Chavanas, Stephane;Adoue, Veronique;Mechin, Marie-Claire;Ying, Shibo;Dong, Sijun;Duplan, Helene;Charveron, Marie;Takahara, Hidenari;Serre, Guy;Simon, Michel
通讯作者：
Simon, Michel
影响因子：
11.4
作者：
CHEVALLIERGRECO, A;MANET, E;SERGEANT, A
通讯作者：
SERGEANT, A
影响因子：
14.9
作者：
Bailey TL;Johnson J;Grant CE;Noble WS
通讯作者：
Noble WS
影响因子：
14.9
作者：
BAYLIS, SA;PURIFOY, DJM;LITTLER, E
通讯作者：
LITTLER, E