Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection.
Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection.
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不孕症和沙眼衣原体感染女性阴道微生物群的变化。
DOI：
10.3389/fcimb.2021.698840
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发表时间：
2021
影响因子：
5.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Chen H;Wang L;Zhao L;Luo L;Min S;Wen Y;Lei W;Shu M;Li Z
沙眼衣原体（C.沙眼）是全世界细菌性传播感染（STI）的最常见病原体，并且如果从女性生殖道的下部上升到上部，则会导致严重的健康后遗症，例如宫颈炎、盆腔炎，甚至不孕症。以前的研究已经揭示了阴道微生物群在性传播感染易感性中的关键作用。然而，在不孕和感染C。沙眼仍然未知。本研究使用宏基因组分析测序的16S rRNA基因扩增子，以检查输卵管性不孕妇女的阴道微生物谱，这些妇女是C。沙眼衣原体阴性者和C.抗生素治疗前后的沙眼阳性。女性是C。将沙眼阴性且被认为健康的人招募作为生态良好和生态失调的参考。患有输卵管不孕症的女性和C。沙眼衣原体感染呈现出独特的以乳杆菌为主的阴道微生物群，而不是以卷曲乳杆菌为主的阴道微生物群，并且表现出乳杆菌属、双歧杆菌属、肠杆菌属、变形菌属和链球菌属的减少，伴随着细胞因子如干扰素（IFN）-γ和白细胞介素（IL）-10水平的降低。这种改变的阴道微生物群可以在C的标准治疗后不同程度地恢复。沙眼这种变化可能是C.沙眼衣原体感染的输卵管不孕症妇女之间，而没有显着差异的门，纲，和操作分类单位（OTU）水平之间的输卵管不孕症的妇女之间观察到的C。沙眼阴性和健康对照。这是第一项提供阴道微生物群与C。沙眼衣原体感染与输卵管不孕症的妇女，并强调了前所未有的潜在机会，预测C。沙眼感染
Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) worldwide and causes serious health sequelae such as cervicitis, pelvic inflammatory disease, and even infertility if ascending from the lower to the upper female genital tract. Previous studies have revealed the pivotal role of vaginal microbiota in susceptibility to STIs. However, alterations in the vaginal microbiota in women who are infertile and infected with C. trachomatis remain unknown. This study used metagenomic analysis of sequenced 16S rRNA gene amplicons to examine the vaginal microbial profiles of women with tubal infertility who were C. trachomatis-negative and those who were C. trachomatis-positive pre- and post-antibiotic treatment. Women who were C. trachomatis-negative and deemed healthy were recruited as references of eubiosis and dysbiosis. Women with tubal infertility and C. trachomatis infection presented a unique Lactobacillus iners-dominated vaginal microbiota rather than one dominated by Lactobacillus crispatus and displayed a decrease in Lactobacillus, Bifidobacterium, Enterobacter, Atopobium, and Streptococcus, accompanied by decreased levels of cytokines such as interferon (IFN)-γ and interleukin (IL)-10. This altered vaginal microbiota could be restored with varying degrees after standard treatment for C. trachomatis. This shift could be a predictive vaginal microbiota signature for C. trachomatis infection among females with tubal infertility, while no significant differences in phylum, class, and operational taxonomic unit (OTU) levels were observed between women with tubal infertility who were C. trachomatis-negative and healthy controls. This is the first study to provide data on the association of vaginal microbiota with C. trachomatis infection among women with tubal infertility and highlights unprecedented potential opportunities to predict C. trachomatis infection.
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影响因子：
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作者：
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