Preovulatory Aging In Vivo and In Vitro Affects Maturation Rates, Abundance of Selected Proteins, Histone Methylation Pattern and Spindle Integrity in Murine Oocytes.
Preovulatory Aging In Vivo and In Vitro Affects Maturation Rates, Abundance of Selected Proteins, Histone Methylation Pattern and Spindle Integrity in Murine Oocytes.
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体内和体外衰老会影响鼠卵母细胞中的成熟率，丰富的蛋白质，组蛋白甲基化模式和纺锤体完整性。
DOI：
10.1371/journal.pone.0162722
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Eichenlaub-Ritter U
中科院分区：
文献类型：
--
作者：
Demond H;Trapphoff T;Dankert D;Heiligentag M;Grümmer R;Horsthemke B;Eichenlaub-Ritter U
延迟排卵和延迟受精可导致卵母细胞发育能力降低。与卵母细胞排卵后老化的后果相反，如果排卵延迟（排卵前老化），则对卵母细胞成熟过程中发生的分子过程几乎一无所知。我们研究了两种排卵前衰老模型中卵母细胞成熟的几个方面：体外卵泡培养和体内小鼠模型，其中使用GnRH拮抗剂西曲瑞克推迟排卵。两种模型均显示出老化后卵母细胞成熟率显著降低。此外，在体外排卵前老化解除调节的母体效应基因Smarca 4和Nlrp5的蛋白质丰度，降低组蛋白H3K9三甲基化的水平，并造成染色体排列和纺锤体构象的重大恶化。YBX2是卵母细胞中mRNA稳定性、储存和募集的重要调节因子，其蛋白丰度不受体外老化的影响。相反，体内排卵前老化导致Ybx2转录和YBX 2蛋白丰度的减少。总之，排卵前衰老似乎影响卵母细胞的各种过程，这可以解释低成熟率和先前描述的受精和胚胎发育失败。
Delayed ovulation and delayed fertilization can lead to reduced developmental competence of the oocyte. In contrast to the consequences of postovulatory aging of the oocyte, hardly anything is known about the molecular processes occurring during oocyte maturation if ovulation is delayed (preovulatory aging). We investigated several aspects of oocyte maturation in two models of preovulatory aging: an in vitro follicle culture and an in vivo mouse model in which ovulation was postponed using the GnRH antagonist cetrorelix. Both models showed significantly reduced oocyte maturation rates after aging. Furthermore, in vitro preovulatory aging deregulated the protein abundance of the maternal effect genes Smarca4 and Nlrp5, decreased the levels of histone H3K9 trimethylation and caused major deterioration of chromosome alignment and spindle conformation. Protein abundance of YBX2, an important regulator of mRNA stability, storage and recruitment in the oocyte, was not affected by in vitro aging. In contrast, in vivo preovulatory aging led to reduction in Ybx2 transcript and YBX2 protein abundance. Taken together, preovulatory aging seems to affect various processes in the oocyte, which could explain the low maturation rates and the previously described failures in fertilization and embryonic development.
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影响因子：
5.2
作者：
Nath S;Ghatak D;Das P;Roychoudhury S
通讯作者：
Roychoudhury S
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影响因子：
10.6
作者：
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影响因子：
3.8
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影响因子：
13.3
作者：
Cortvrindt, RG;Smitz, JEJ
通讯作者：
Smitz, JEJ
影响因子：
2.7
作者：
Kim, Boram;Zhang, Xuesen;Kan, Rui;Cohen, Roy;Mukai, Chinatsu;Travis, Alexander J.;Coonrod, Scott A.
通讯作者：
Coonrod, Scott A.