Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green.
Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green.
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DOI：
10.1038/srep12080
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发表时间：
2015-07-13
影响因子：
4.6
通讯作者：
Mimori K
中科院分区：
文献类型：
--
作者：
Ueo H;Shinden Y;Tobo T;Gamachi A;Udo M;Komatsu H;Nambara S;Saito T;Ueda M;Hirata H;Sakimura S;Takano Y;Uchi R;Kurashige J;Akiyoshi S;Iguchi T;Eguchi H;Sugimachi K;Kubota Y;Kai Y;Shibuta K;Kijima Y;Yoshinaka H;Natsugoe S;Mori M;Maehara Y;Sakabe M;Kamiya M;Kakareka JW;Pohida TJ;Choyke PL;Kobayashi H;Ueo H;Urano Y;Mimori K
基于γ-谷氨酰转肽酶（γ-glutamyltranspeptidase， GGT）在多种癌细胞细胞膜上过表达，而在正常组织中不表达的事实，我们之前开发了γ-谷氨酰羟甲基罗丹明绿（glu - hmrg）作为检测活癌细胞的工具。GGT切割探针产生绿色荧光。在此，我们探讨了glu - hmrg在保乳手术中临床应用的可行性。我们发现，glu - hmrg切割产生的荧光可以很容易地从正常乳腺组织中区分乳腺肿瘤，即使是小于1mm的肿瘤，在应用后仅5分钟内，灵敏度为92%，特异性为94%。我们相信这种快速、低成本的方法代表了保乳手术术中边缘评估的突破。
We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.
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影响因子：
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